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PMID: 8196607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A carbon source-responsive promoter element necessary for activation of the isocitrate lyase gene ICL1 is common to genes of the gluconeogenic pathway in the yeast Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 3613-22

Schöler A, Schüller HJ

Abstract

The expression of yeast genes encoding gluconeogenic enzymes depends strictly on the carbon source available in the growth medium. We have characterized the control region of the isocitrate lyase gene ICL1, which is derepressed more than 200-fold after transfer of cells from fermentative to nonfermentative growth conditions. Deletion analysis of the ICL1 promoter led to the identification of an upstream activating sequence element, UASICL1 (5' CATTCATCCG 3'), necessary and sufficient for conferring carbon source-dependent regulation on a heterologous reporter gene. Similar sequence motifs were also found in the upstream regions of coregulated genes involved in gluconeogenesis. This carbon source-responsive element (CSRE) interacts with a protein factor, designated Ang1 (activator of nonfermentative growth), detectable only in extracts derived from derepressed cells. Gene activation mediated by the CSRE requires the positively acting derepression genes CAT1 (= SNF1 and CCR1) and CAT3 (= SNF4). In the respective mutants, Ang1-CSRE interaction was no longer observed under repressing or derepressing conditions. Since binding of Ang1 factor to the CSRE could be competed for by an upstream sequence derived from the fructose-1,6-bisphosphatase gene FBP1, we propose that the CSRE functions as a UAS element common to genes of the gluconeogenic pathway.

Related Genes
ADH2 BEM1 CAT1 CAT3 CCR1 CIT2 CLB3 DAF1 FBP1 GAL2 HXT1 ICL1 ILV2 PCK1 SLY2 SNF1 SNF4 SRP1 TIF1
MeSH Terms
Base Sequence Carbohydrate Metabolism Consensus Sequence DNA-Binding Proteins/metabolism Fructose-Bisphosphatase/genetics Gene Deletion Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Genotype Gluconeogenesis/genetics Isocitrate Lyase/biosynthesis,genetics Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Oligonucleotide Probes Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/enzymology,genetics,metabolism Transcriptional Activation
Chemicals
DNA-Binding Proteins Oligonucleotide Probes Fructose-Bisphosphatase Isocitrate Lyase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schöler A
Institut für Mikrobiologie, Biochemie und Genetik, Universität Erlangen/Nürnberg, Germany.
Schüller H J
References (70)
70 references, click to expand
  1. Short repeated elements in the upstream regulatory region of the SUC2 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jul;6(7):2324-33 PMID: 3097512
  2. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  3. Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response.
    EMBO J. 1991 Nov;10(11):3373-7 PMID: 1915298
  4. Characterization of trans-acting mutations affecting Ty and Ty-mediated transcription in Saccharomyces cerevisiae.
    Curr Genet. 1991 Dec;20(6):441-8 PMID: 1664298
  5. Alteration by phenobarbital and 3-methyl-cholanthrene of functional and structural changes in rat liver due to carbon tetrachloride inhalation.
    J Pharmacol Exp Ther. 1975 Apr;193(1):281-92 PMID: 1133769
  6. Glucose repression in the yeast Saccharomyces cerevisiae.
    Mol Microbiol. 1992 Jan;6(1):15-21 PMID: 1310793
  7. Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae.
    Curr Genet. 1993 May-Jun;23(5-6):375-81 PMID: 8319292
  8. Genetics of carbon catabolite repression in Saccharomycess cerevisiae: genes involved in the derepression process.
    Mol Gen Genet. 1977 Feb 28;151(1):95-103 PMID: 194140
  9. Regulatory regions in the yeast FBP1 and PCK1 genes.
    FEBS Lett. 1992 Oct 19;311(2):110-4 PMID: 1327878
  10. Recessive mutations conferring resistance to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1983 Mar;153(3):1405-14 PMID: 6337998
  11. Control of mRNA turnover as a mechanism of glucose repression in Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Jul;12(7):2941-8 PMID: 1620107
  12. Yeast SNF2/SWI2, SNF5, and SNF6 proteins function coordinately with the gene-specific transcriptional activators GAL4 and Bicoid.
    Genes Dev. 1992 Sep;6(9):1707-15 PMID: 1516829
  13. Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeast.
    Eur J Biochem. 1991 Sep 1;200(2):311-9 PMID: 1889400
  14. New genes involved in carbon catabolite repression and derepression in the yeast Saccharomyces cerevisiae.
    J Bacteriol. 1982 Sep;151(3):1123-8 PMID: 7050076
  15. Fructose bisphosphatase of Saccharomyces cerevisiae. Cloning, disruption and regulation of the FBP1 structural gene.
    J Mol Biol. 1985 Nov 20;186(2):307-19 PMID: 3003364
  16. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  17. SRN1, a yeast gene involved in RNA processing, is identical to HEX2/REG1, a negative regulator in glucose repression.
    Mol Cell Biol. 1992 Jun;12(6):2673-80 PMID: 1588964
  18. Importance of a flanking AT-rich region in target site recognition by the GC box-binding zinc finger protein MIG1.
    Mol Cell Biol. 1994 Mar;14(3):1979-85 PMID: 8114729
  19. Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein.
    Mol Cell Biol. 1989 Nov;9(11):5034-44 PMID: 2557546
  20. Adjacent upstream activation sequence elements synergistically regulate transcription of ADH2 in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Jan;9(1):34-42 PMID: 2648133
  21. A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids.
    Gene. 1993 Sep 30;132(1):49-55 PMID: 8406042
  22. Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.
    Mol Cell Biol. 1988 Feb;8(2):647-54 PMID: 2832731
  23. Regulation of transcription of the gene coding for peroxisomal 3-oxoacyl-CoA thiolase of Saccharomyces cerevisiae.
    Eur J Biochem. 1991 Aug 15;200(1):113-22 PMID: 1715273
  24. Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Aug;13(8):4999-5009 PMID: 8393142
  25. Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations.
    Genetics. 1987 Feb;115(2):247-53 PMID: 3549450
  26. Structural analysis of the 5' regions of yeast SUC genes revealed analogous palindromes in SUC, MAL and GAL.
    Mol Gen Genet. 1988 Mar;211(3):446-54 PMID: 2835632
  27. GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats.
    Mol Cell Biol. 1991 Oct;11(10):5101-12 PMID: 1922034
  28. Genetic and biochemical evidence for hexokinase PII as a key enzyme involved in carbon catabolite repression in yeast.
    Mol Gen Genet. 1980;178(3):633-7 PMID: 6993859
  29. Identification of the upstream activating sequence of MAL and the binding sites for the MAL63 activator of Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Jul;10(7):3797-800 PMID: 2192262
  30. Transcriptional regulation of the yeast cytochrome c gene.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627-31 PMID: 226972
  31. Genetic analysis of serine biosynthesis and glucose repression in yeast.
    Curr Genet. 1992 Apr;21(4-5):295-300 PMID: 1326413
  32. A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis.
    Cell. 1990 Jan 26;60(2):307-17 PMID: 2404612
  33. Glucose repression in Saccharomyces cerevisiae is directly associated with hexose phosphorylation by hexokinases PI and PII.
    Eur J Biochem. 1991 Aug 1;199(3):511-8 PMID: 1868842
  34. Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae.
    Genetics. 1984 Dec;108(4):845-58 PMID: 6392017
  35. Isolation and expression analysis of two yeast regulatory genes involved in the derepression of glucose-repressible enzymes.
    Mol Gen Genet. 1987 Sep;209(2):366-73 PMID: 2823078
  36. The organization and transcription of the galactose gene cluster of Saccharomyces.
    J Mol Biol. 1981 Oct 25;152(2):285-315 PMID: 6276569
  37. Extragenic suppressors of yeast glucose derepression mutants leading to constitutive synthesis of several glucose-repressible enzymes.
    J Bacteriol. 1991 Mar;173(6):2045-52 PMID: 2002006
  38. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  39. A yeast gene that is essential for release from glucose repression encodes a protein kinase.
    Science. 1986 Sep 12;233(4769):1175-80 PMID: 3526554
  40. Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;193(3):507-12 PMID: 6323921
  41. The GAM1/SNF2 gene of Saccharomyces cerevisiae encodes a highly charged nuclear protein required for transcription of the STA1 gene.
    Mol Gen Genet. 1991 Aug;228(1-2):270-80 PMID: 1886612
  42. The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins.
    Mol Cell Biol. 1991 Feb;11(2):699-704 PMID: 1899286
  43. Carbon catabolite repression in yeast.
    Eur J Biochem. 1992 Jun 1;206(2):297-313 PMID: 1597176
  44. Proteinases, proteolysis and biological control in the yeast Saccharomyces cerevisiae.
    Yeast. 1985 Dec;1(2):139-57 PMID: 3916861
  45. A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast.
    Genetics. 1984 May;107(1):19-32 PMID: 6373495
  46. Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription.
    Nucleic Acids Res. 1992 Oct 25;20(20):5271-8 PMID: 1437546
  47. Pleiotropic Mutations at the TUP1 Locus That Affect the Expression of Mating-Type-Dependent Functions in SACCHAROMYCES CEREVISIAE.
    Genetics. 1980 Apr;94(4):899-920 PMID: 17249022
  48. Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
    Cell. 1992 Feb 7;68(3):573-83 PMID: 1339306
  49. AAR1/TUP1 protein, with a structure similar to that of the beta subunit of G proteins, is required for a1-alpha 2 and alpha 2 repression in cell type control of Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Jul;11(7):3773-9 PMID: 1904546
  50. Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection.
    Trends Genet. 1992 Nov;8(11):387-91 PMID: 1332230
  51. Isolation of Saccharomyces cerevisiae mutants constitutive for invertase synthesis.
    J Bacteriol. 1986 Jun;166(3):1123-7 PMID: 3519577
  52. Cloning and characterization of the CYC8 gene mediating glucose repression in yeast.
    Gene. 1988 Dec 15;73(1):97-111 PMID: 2854095
  53. Mutants of yeast defective in sucrose utilization.
    Genetics. 1981 May;98(1):25-40 PMID: 7040163
  54. The ICL1 gene from Saccharomyces cerevisiae.
    Eur J Biochem. 1992 Mar 15;204(3):983-90 PMID: 1551398
  55. A protein kinase substrate identified by the two-hybrid system.
    Science. 1992 Jul 31;257(5070):680-2 PMID: 1496382
  56. Isolation of a catabolite repression mutant of yeast as a revertant of a strain that is maltose negative in the respiratory-deficient state.
    J Bacteriol. 1975 Mar;121(3):747-52 PMID: 163813
  57. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.
    Microbiol Rev. 1987 Dec;51(4):458-76 PMID: 2830478
  58. Molecular characterization of yeast regulatory gene CAT3 necessary for glucose derepression and nuclear localization of its product.
    Gene. 1988 Jul 30;67(2):247-57 PMID: 3049255
  59. Identification of UAS elements and binding proteins necessary for derepression of Saccharomyces cerevisiae fructose-1,6-bisphosphatase.
    Curr Genet. 1992 Nov;22(5):363-70 PMID: 1330335
  60. A yeast mutation that stabilizes a plasmid bearing a mutated ARS1 element.
    Mol Cell Biol. 1989 Feb;9(2):809-16 PMID: 2651904
  61. Genes required for derepression of an extracellular glucoamylase gene, STA2, in the yeast Saccharomyces.
    Yeast. 1993 May;9(5):533-41 PMID: 8322516
  62. The UAS(MAL) is a bidirectional promotor element required for the expression of both the MAL61 and MAL62 genes of the Saccharomyces MAL6 locus.
    Curr Genet. 1992 Sep;22(3):181-9 PMID: 1525871
  63. Characterization of a transcriptional control element involved in proliferation of peroxisomes in yeast in response to oleate.
    Eur J Biochem. 1993 May 15;214(1):323-31 PMID: 8508802
  64. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  65. ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1.
    Mol Cell Biol. 1993 Jul;13(7):4391-9 PMID: 8321238
  66. Ssn6-Tup1 is a general repressor of transcription in yeast.
    Cell. 1992 Feb 21;68(4):709-19 PMID: 1739976
  67. The yeast regulatory protein ADR1 binds in a zinc-dependent manner to the upstream activating sequence of ADH2.
    Mol Cell Biol. 1988 Oct;8(10):4552-6 PMID: 3141794
  68. Coordinate genetic control of yeast fatty acid synthase genes FAS1 and FAS2 by an upstream activation site common to genes involved in membrane lipid biosynthesis.
    EMBO J. 1992 Jan;11(1):107-14 PMID: 1740101
  69. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins.
    EMBO J. 1990 Sep;9(9):2891-8 PMID: 2167835
  70. Glycolytic enzymes and intermediates in carbon catabolite repression mutants of Saccharomyces cerevisiae.
    Mol Gen Genet. 1980 Jan;177(2):345-50 PMID: 6988675
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.3613-3622.1994
Published
1994-06-00
Pages
3613-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358729
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.6.3613-3622.1994 PMC Full Text View journal details
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