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PMID: 1437546 Published · ppublish English Journal Article

Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription.

Nucleic acids research ·Vol. 20 ·No. 20 ·1992-10-25 ·Pages 5271-8

Nehlin JO, Carlberg M, Ronne H

Abstract

We have cloned a yeast gene, SKO1, which in high copy number suppresses lethal overexpression of cAMP-dependent protein kinase. SKO1 encodes a bZIP protein that binds to the CRE motif, TGACGTCA. We found that SKO1 also binds to a CRE-like site in SUC2, a yeast gene encoding invertase which is under positive control by cAMP. A disruption of the SKO1 gene causes a partial derepression of SUC2, indicating that SKO1 is a negative regulator of the SUC2 gene. SKO1 interacts positively with MIG1, a zinc finger protein that mediates glucose repression of SUC2. A kinetic analysis revealed a complex regulation of the SUC2 mRNA in response to glucose. First, MIG1 mediates a rapid and strong repression of SUC2, which is complete within 10 minutes. Second, a MIG1-independent process causes a further slow reduction in the mRNA. Third, in the absence of MIG1, there is also a rapid but transient glucose induction of the SUC2 mRNA. This induction is correlated with a transient loss of SKO1-dependent repression.

MeSH Terms
Amino Acid Sequence Base Sequence Basic-Leucine Zipper Transcription Factors Binding Sites/genetics Blotting, Northern Cloning, Molecular DNA-Binding Proteins/chemistry,genetics,metabolism G-Box Binding Factors Gene Expression Regulation, Fungal/drug effects,physiology Genes, Fungal/genetics Glucose/pharmacology Glucosyltransferases/genetics Leucine Zippers/genetics Molecular Sequence Data Oligonucleotide Probes/genetics Plant Proteins/chemistry,genetics,metabolism Plasmids/genetics Promoter Regions, Genetic/genetics Repressor Proteins/chemistry,genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/chemistry,genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors Transcription, Genetic/genetics
Chemicals
Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins G-Box Binding Factors Oligonucleotide Probes Plant Proteins Repressor Proteins SKO1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Glucosyltransferases trehalose-6-phosphate synthase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nehlin J O
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Carlberg M
Ronne H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.20.5271
Published
1992-10-25
Pages
5271-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334331
Subset
IM
Databases
GENBANK
X67875
External Links
PubMed source DOI: 10.1093/nar/20.20.5271 PMC Full Text View journal details
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