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PMID: 8223459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential.

The EMBO journal ·Vol. 12 ·No. 12 ·1993-12-00 ·Pages 4489-98

Shimazaki T, Okazawa H, Fujii H, Ikeda M, Tamai K, McKay RD, Muramatsu M, Hamada H

Abstract

The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma cells (Oct-3+) and fibroblasts (Oct-3-), and have studied the regulation and function of Oct-3. Upon fusion, the hybrid cells differentiated to nestin+/Brn-2+ cells resembling neuroepithelial stem cells. Expression of Oct-3 was extinguished at the transcriptional level in all the hybrid cells examined. The Oct-3 modulating activity required for the Oct-3-mediated enhancer activation was also extinguished. When the Oct-3 transactivating function was introduced into the hybrid cells, they transformed into morphologically distinct nestin-/Brn-2- cells ('revertants'). When the 'revertant' cells subsequently lost Oct-3 expression, they differentiated back to nestin+/Brn-2+ cells. The close correlation between the phenotypic changes and the gain/loss of Oct-3 function indicates that Oct-3 can induce dedifferentiation of the neural cells.

Related Genes
Oct-3
MeSH Terms
Animals Base Sequence Blotting, Northern Cell Differentiation/genetics,physiology DNA DNA-Binding Proteins/genetics,physiology Embryonal Carcinoma Stem Cells Hybrid Cells/cytology L Cells Mice Molecular Sequence Data Neoplastic Stem Cells/cytology Octamer Transcription Factor-3 Phenotype Transcription Factors/genetics,physiology Transcriptional Activation
Chemicals
DNA-Binding Proteins Octamer Transcription Factor-3 Pou5f1 protein, mouse Transcription Factors DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Shimazaki T
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Okazawa H
Fujii H
Ikeda M
Tamai K
McKay R D
Muramatsu M
Hamada H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-12-00
Pages
4489-98
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413873
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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