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PMID: 8234251 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amino acid sequence of a putative sodium channel expressed in the giant axon of the squid Loligo opalescens.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 21 ·1993-11-01 ·Pages 10026-30

Rosenthal JJ, Gilly WF

Abstract

A full-length cDNA encoding a putative Na+ channel (GFLN1) has been cloned from a library prepared from the stellate ganglion of Loligo opalescens. The cDNA encodes a predicted protein of 1784 amino acids. Regions of the GFLN1 protein with defined functional importance (membrane span S4, the SS1 and SS2 segments, and interdomain III-IV) are highly conserved among all vertebrate Na+ channel alpha-subunit structures. Northern blot hybridization and RNase protection assays verify that mRNA corresponding to GFLN1 is expressed in neurons of the giant fiber lobe that form the giant axon. We propose that GFLN1 encodes the Na+ channel that has been extensively studied in the squid axon.

MeSH Terms
Amino Acid Sequence Animals Axons/metabolism Base Sequence Conserved Sequence DNA Primers DNA, Complementary Decapodiformes/metabolism Electrophorus Gene Expression Gene Library Molecular Sequence Data Polymerase Chain Reaction RNA, Antisense RNA, Messenger/biosynthesis,isolation & purification Rats Sequence Homology, Amino Acid Sodium Channels/biosynthesis,chemistry Stellate Ganglion/metabolism
Chemicals
DNA Primers DNA, Complementary GFLN1 protein, Loligo opalescens RNA, Antisense RNA, Messenger Sodium Channels
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rosenthal J J
Hopkins Marine Station, Stanford University, Department of Biological Sciences, Pacific Grove, CA 93950-3094.
Gilly W F
References (31)
31 references, click to expand
  1. Structural parts involved in activation and inactivation of the sodium channel.
    Nature. 1989 Jun 22;339(6226):597-603 PMID: 2543931
  2. Molecular analysis of the para locus, a sodium channel gene in Drosophila.
    Cell. 1989 Sep 22;58(6):1143-54 PMID: 2550145
  3. Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):8170-4 PMID: 2554302
  4. Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
    Neuron. 1989 Jul;3(1):33-49 PMID: 2559760
  5. Primary structure and expression of a sodium channel characteristic of denervated and immature rat skeletal muscle.
    Neuron. 1990 Feb;4(2):233-42 PMID: 2155010
  6. Alterations in the expression and gating of Drosophila sodium channels by mutations in the para gene.
    Neuron. 1989 Apr;2(4):1301-11 PMID: 2560637
  7. Jet-propelled escape in the squid Loligo opalescens: concerted control by giant and non-giant motor axon pathways.
    Proc Natl Acad Sci U S A. 1990 Apr;87(8):2911-5 PMID: 2326255
  8. Control of the spatial distribution of sodium channels in giant fiber lobe neurons of the squid.
    Neuron. 1990 Nov;5(5):663-74 PMID: 2171590
  9. Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents.
    J Neurosci. 1991 Apr;11(4):918-27 PMID: 2010814
  10. 'Touchdown' PCR to circumvent spurious priming during gene amplification.
    Nucleic Acids Res. 1991 Jul 25;19(14):4008 PMID: 1861999
  11. Single-channel, macroscopic, and gating currents from sodium channels in the squid giant axon.
    Biophys J. 1991 Dec;60(6):1499-510 PMID: 1663795
  12. Alternatively spliced sodium channel transcripts in brain and muscle.
    J Neurosci. 1992 Apr;12(4):1370-81 PMID: 1313493
  13. Calcium channel characteristics conferred on the sodium channel by single mutations.
    Nature. 1992 Apr 2;356(6368):441-3 PMID: 1313551
  14. Molecular cloning of an atypical voltage-gated sodium channel expressed in human heart and uterus: evidence for a distinct gene family.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4893-7 PMID: 1317577
  15. Primary structure of squid sodium channel deduced from the complementary DNA sequence.
    Biochem Biophys Res Commun. 1992 Jul 15;186(1):61-8 PMID: 1339273
  16. Deduced amino acid sequence of a putative sodium channel from the scyphozoan jellyfish Cyanea capillata.
    Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7419-23 PMID: 8394021
  17. A quantitative description of membrane current and its application to conduction and excitation in nerve.
    J Physiol. 1952 Aug;117(4):500-44 PMID: 12991237
  18. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  19. Sodium channels and gating currents.
    Physiol Rev. 1981 Jul;61(3):644-83 PMID: 6265962
  20. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  21. A method for isolation of intact, translationally active ribonucleic acid.
    DNA. 1983;2(4):329-35 PMID: 6198133
  22. Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
    Nature. 1984 Nov 8-14;312(5990):121-7 PMID: 6209577
  23. Existence of distinct sodium channel messenger RNAs in rat brain.
    Nature. 1986 Mar 13-19;320(6058):188-92 PMID: 3754035
  24. An integrated view of the molecular toxinology of sodium channel gating in excitable cells.
    Annu Rev Neurosci. 1987;10:237-67 PMID: 2436544
  25. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  26. Modulation of sodium-channel mRNA levels in rat skeletal muscle.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8721-5 PMID: 2446331
  27. In vitro RNA synthesis with SP6 RNA polymerase.
    Methods Enzymol. 1987;155:397-415 PMID: 2828872
  28. Selective induction of brain type II Na+ channels by nerve growth factor.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):924-8 PMID: 2448784
  29. Primary structure of rat brain sodium channel III deduced from the cDNA sequence.
    FEBS Lett. 1988 Feb 8;228(1):187-94 PMID: 2449363
  30. Probing the molecular structure of the voltage-dependent sodium channel.
    Annu Rev Neurosci. 1988;11:455-95 PMID: 2452597
  31. The scanning model for translation: an update.
    J Cell Biol. 1989 Feb;108(2):229-41 PMID: 2645293
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.21.10026
Published
1993-11-01
Pages
10026-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47706
Subset
IM
Grants
NINDS NIH HHS · NS17510 · United States
Databases
GENBANK
L19979
External Links
PubMed source DOI: 10.1073/pnas.90.21.10026 PMC Full Text View journal details
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