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PMID: 8244005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic and molecular analysis of the X chromosomal region 14B17-14C4 in Drosophila melanogaster: loss of function in NONA, a nuclear protein common to many cell types, results in specific physiological and behavioral defects.

Genetics ·Vol. 135 ·No. 2 ·1993-10-00 ·Pages 419-42

Stanewsky R, Rendahl KG, Dill M, Saumweber H

Abstract

We have performed a genetic analysis of the 14C region of the X chromosome of Drosophila melanogaster to isolate loss of function alleles of no-on-transient A (nonA; 14C1-2; 1-52.3). NONA is a nuclear protein common to many cell types, which is present in many puffs on polytene chromosomes. Sequence data suggest that the protein contains a pair of RNA binding motifs (RRM) found in many single-strand nucleic acid binding proteins. Hypomorphic alleles of this gene, which lead to aberrant visual and courtship song behavior, still contain normally distributed nonA RNA and NONA protein in embryos, and in all available alleles NONA protein is present in puffs of third instar larval polytene chromosomes. We find that complete loss of this general nuclear protein is semilethal in hemizygous males and homozygous cell lethal in the female germline. Surviving males show more extreme defects in nervous system function than have been described for the hypomorphic alleles. Five other essential genes that reside within this region have been partially characterized.

MeSH Terms
Alleles Animals Animals, Genetically Modified Base Sequence Chromosome Mapping Chromosomes/ultrastructure Crosses, Genetic DNA/chemistry DNA Primers Drosophila Proteins Drosophila melanogaster/embryology,genetics,radiation effects Embryo, Nonmammalian/physiology Female Genes, Lethal Genetic Complementation Test Male Molecular Sequence Data Mutagenesis Nuclear Proteins/genetics,physiology Organ Specificity Phenotype Polymerase Chain Reaction X Chromosome/radiation effects X-Rays Zygote
Chemicals
DNA Primers Drosophila Proteins NONA protein, Drosophila Nuclear Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stanewsky R
Institut für Entwicklungsbiologie, Universität zu Köln, Germany.
Rendahl K G
Dill M
Saumweber H
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.2.419
Published
1993-10-00
Pages
419-42
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205646
Subset
IM
Grants
NIGMS NIH HHS · GM-21473 · United States
NCHM CDC HHS · HM1014701 · United States
External Links
PubMed source DOI: 10.1093/genetics/135.2.419 PMC Full Text View journal details
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