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PMID: 8244921 Published · ppublish English Comparative Study Journal Article

Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis.

Journal of bacteriology ·Vol. 175 ·No. 23 ·1993-12-00 ·Pages 7523-32

Tynkkynen S, Buist G, Kunji E, Kok J, Poolman B, Venema G, Haandrikman A

Abstract

The nucleotide sequence of a chromosomal DNA fragment of Lactococcus lactis subsp. lactis SSL135, previously implicated in peptide utilization, has been determined. The genes oppDFBCA, encoding the oligopeptide transport system (Opp), and that encoding the endopeptidase PepO were located on this 8.9-kb DNA fragment. The oppDFBCA and pepO genes are probably organized in an operon. Analysis of the deduced amino acid sequences of the genes indicated that the oligopeptide transport system consists of two ATP-binding proteins OppD and OppF, two integral membrane proteins OppB and OppC, and a substrate-binding protein OppA. On the basis of the homology of OppF and OppD of L. lactis with other ABC (ATP-binding cassette) transporter proteins, the L. lactis Opp system can be classified as a member of this group. Two integration mutants, one defective in OppA and the other defective in PepO, were constructed. Growth of these mutants in a chemically defined medium with oligopeptides showed that the transport system, but not the endopeptidase, is essential for the utilization of peptides longer than three residues. Uptake of the pentapeptide Leu-enkephalin in glycolyzing lactococcal cells was followed by rapid hydrolysis of the peptide intracellularly. Importantly, extracellular hydrolysis of Leu-enkephalin is not observed. The OppA-deficient mutant was unable to transport Leu-enkephalin. Growth experiments with pasteurized milk revealed that transport of oligopeptides forms an essential part of the proteolytic system in lactococci.

Related Genes
oppA oppB oppC oppD oppF pepO
MeSH Terms
Amino Acid Sequence Amino Acids/metabolism Animals Bacterial Proteins/genetics Base Sequence Biological Transport Carrier Proteins/genetics Genes, Bacterial Glycolysis Lactococcus lactis/genetics,growth & development,metabolism Lipoproteins/genetics Metalloendopeptidases/genetics Milk/metabolism Molecular Sequence Data Mutagenesis, Insertional Oligopeptides/metabolism Plasmids/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Amino Acids Bacterial Proteins Carrier Proteins Lipoproteins Oligopeptides oligopeptide-binding protein, bacteria Metalloendopeptidases oligopeptidase PepO
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tynkkynen S
Research and Development Centre, Valio Ltd., Helsinki, Finland.
Buist G
Kunji E
Kok J
Poolman B
Venema G
Haandrikman A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.23.7523-7532.1993
Published
1993-12-00
Pages
7523-32
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206908
Subset
IM
Databases
GENBANK
L18760
External Links
PubMed source DOI: 10.1128/jb.175.23.7523-7532.1993 PMC Full Text View journal details
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