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PMID: 8246972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Saccharomyces cerevisiae Cdc68 transcription activator is antagonized by San1, a protein implicated in transcriptional silencing.

Molecular and cellular biology ·Vol. 13 ·No. 12 ·1993-12-00 ·Pages 7553-65

Xu Q, Johnston GC, Singer RA

Abstract

The CDC68 gene (also called SPT16) encodes a transcription factor for the expression of a diverse set of genes in the budding yeast Saccharomyces cerevisiae. To identify other proteins that are functionally related to the Cdc68 protein, we searched for genetic suppressors of a cdc68 mutation. Four suppressor genes in which mutations reverse the temperature sensitivity imposed by the cdc68-1 mutation were found. We show here that one of the suppressor genes is the previously reported SAN1 gene; san1 mutations were originally identified as suppressors of a sir4 mutation, implicated in the chromatin-mediated transcriptional silencing of the two mating-type loci HML and HMR. Each san1 mutation, including a san1 null allele, reversed all aspects of the cdc68 mutant phenotype. Conversely, increased copy number of the wild-type SAN1 gene lowered the restrictive temperature for the cdc68-1 mutation. Our findings suggest that the San1 protein antagonizes the transcriptional activator function of the Cdc68 protein. The identification of san1 mutations as suppressors of cdc68 mutations suggests a role for Cdc68 in chromatin structure.

Related Genes
CDC68 SAN1 SPT16
MeSH Terms
Alleles Cell Cycle Proteins Cyclins/genetics Fungal Proteins/genetics Genes, Fungal Genes, Mating Type, Fungal Genes, Suppressor Histones/genetics Mutation Phenotype Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Trans-Activators/genetics Transcription Factors Transcription, Genetic Transcriptional Elongation Factors
Chemicals
Cell Cycle Proteins Cyclins Fungal Proteins Histones SPT16 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Transcriptional Elongation Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xu Q
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Johnston G C
Singer R A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.12.7553-7565.1993
Published
1993-12-00
Pages
7553-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364827
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.12.7553-7565.1993 PMC Full Text View journal details
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