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PMID: 8248179 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Brn-3.0: a POU-domain protein expressed in the sensory, immune, and endocrine systems that functions on elements distinct from known octamer motifs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 22 ·1993-11-15 ·Pages 10841-5

Gerrero MR, McEvilly RJ, Turner E, Lin CR, O'Connell S, Jenne KJ, Hobbs MV, Rosenfeld MG

Abstract

Characterization of Brn-3.0 and identification of a highly related member (Brn-3.1) of the class IV POU-domain family suggest potential roles of Brn-3.0 in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3.0 is expressed in the pituitary gland and in a corticotroph cell line. A functional DNA response element has been identified in the proopiomelanocortin promoter. In contrast to previously described mammalian POU-domain proteins, Brn-3.0 binds relatively ineffectively to known octamer DNA motifs, but instead binds with high affinity to a distinct set of DNA elements, functioning as a transcriptional activator. Brn-3.0, Brn-3.1, and the Drosophila tI-POU share an N-terminal region of homology, referred to as the "POU-IV box," which is similar to a conserved functional domain in the c-myc gene family.

Related Genes
Brn 3.0 Brn 3.1 myc tI-POU unc-86
MeSH Terms
Age Factors Animals Base Sequence Cloning, Molecular DNA Mutational Analysis DNA Primers/chemistry DNA, Complementary/genetics DNA-Binding Proteins/genetics Endocrine Glands/embryology,metabolism Gene Expression Genes Lymphoid Tissue/embryology,metabolism Mice/embryology Molecular Sequence Data Multigene Family Nerve Tissue Proteins/genetics RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Sequence Alignment Sequence Homology, Amino Acid Structure-Activity Relationship Transcription Factor Brn-3 Transcription Factor Brn-3A Transcription Factors/genetics
Chemicals
DNA Primers DNA, Complementary DNA-Binding Proteins Nerve Tissue Proteins POU4F1 protein, human Pou4f1 protein, mouse RNA, Messenger Transcription Factor Brn-3 Transcription Factor Brn-3A Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gerrero M R
Eukaryotic Regulatory Biology Program, Howard Hughes Medical Institute, University of California, San Diego.
McEvilly R J
Turner E
Lin C R
O'Connell S
Jenne K J
Hobbs M V
Rosenfeld M G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.22.10841
Published
1993-11-15
Pages
10841-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47874
Subset
IM
Grants
NIA NIH HHS · AG09822 · United States
NIMH NIH HHS · MH47137 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.22.10841 PMC Full Text View journal details
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