Home LiteratureArticle Details
PMID: 8248209 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and centrosomal localization of human caltractin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11039-43

Lee VD, Huang B

Abstract

Caltractin, a 20-kDa calcium-binding protein, was previously purified and cloned at the DNA level from the unicellular green alga Chlamydomonas. It is a structural component of the basal body complex, the major microtubule-organizing center in Chlamydomonas and the functional homolog of the centrosome in the animal cell. Here we report the characterization of a cDNA encoding a human caltractin that shares a high degree of amino acid identity (70%) with its algal counterpart. Caltractin was identified in both HeLa and BHK cells as a 21-kDa polypeptide specifically localized to the centrosome of interphase and mitotic cells. The high level of conservation in the amino acid sequence of caltractin from algae to humans and its association with the major microtubule-organizing center in the cell suggest that caltractin plays a fundamental role in microtubule-organizing center structure and function.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Calcium-Binding Proteins/metabolism Cell Compartmentation Chromosomal Proteins, Non-Histone Cloning, Molecular Cricetinae Cytoplasm/metabolism,ultrastructure Gene Expression Genes HeLa Cells Humans Molecular Sequence Data Precipitin Tests RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity
Chemicals
Calcium-Binding Proteins Chromosomal Proteins, Non-Histone RNA, Messenger caltractin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee V D
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.
Huang B
References (28)
28 references, click to expand
  1. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation.
    J Cell Biol. 1985 Nov;101(5 Pt 1):1903-12 PMID: 4055898
  2. Two different monoclonal antibodies to alpha-tubulin inhibit the bending of reactivated sea urchin spermatozoa.
    Cell Motil. 1982;2(6):599-614 PMID: 6892013
  3. Isolation and nucleotide sequence of a cDNA encoding human calmodulin.
    Biochem Int. 1984 Aug;9(2):177-85 PMID: 6385987
  4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  5. Purification and characterization of a basal body-associated Ca2+-binding protein.
    J Cell Biol. 1988 Jul;107(1):121-31 PMID: 3292538
  6. Beta-tubulin mutants of the unicellular green alga Chlamydomonas reinhardtii.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):131-5 PMID: 3422409
  7. A human centrosomal protein is immunologically related to basal body-associated proteins from lower eucaryotes and is involved in the nucleation of microtubules.
    J Cell Biol. 1991 Oct;115(1):129-40 PMID: 1918132
  8. The chromosome cycle and the centrosome cycle in the mitotic cycle.
    Int Rev Cytol. 1987;100:49-92 PMID: 3549609
  9. Isolation and characterization of a cDNA clone encoding the 60-kD component of the human SS-A/Ro ribonucleoprotein autoantigen.
    J Clin Invest. 1989 Apr;83(4):1284-92 PMID: 2649513
  10. Centrosome organization and centriole architecture: their sensitivity to divalent cations.
    J Struct Biol. 1992 Mar-Apr;108(2):107-28 PMID: 1486002
  11. Genomic structure of Chlamydomonas caltractin. Evidence for intron insertion suggests a probable genealogy for the EF-hand superfamily of proteins.
    J Mol Biol. 1991 Sep 5;221(1):175-91 PMID: 1920403
  12. An Atriplex nummularia cDNA with Sequence Relatedness to the Algal Caltractin Gene.
    Plant Physiol. 1992 Aug;99(4):1734-5 PMID: 16669106
  13. Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.
    J Cell Biol. 1990 Jun;110(6):2033-42 PMID: 2190990
  14. Localization of the centrin-related 165,000-Mr protein of PtK2 cells during the cell cycle.
    Cell Motil Cytoskeleton. 1991;18(1):1-14 PMID: 2004430
  15. Evolution of EF-hand calcium-modulated proteins. II. Domains of several subfamilies have diverse evolutionary histories.
    J Mol Evol. 1992 May;34(5):416-48 PMID: 1602495
  16. Calcium-induced contraction of the rhizoplast of a quadriflagellate green alga.
    Science. 1978 Dec 1;202(4371):975-7 PMID: 17798796
  17. Microtubule organizing centers.
    Annu Rev Cell Biol. 1985;1:145-72 PMID: 3916316
  18. Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas.
    J Cell Biol. 1992 Dec;119(6):1613-24 PMID: 1361488
  19. Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+-binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product.
    J Cell Biol. 1988 Jul;107(1):133-40 PMID: 2839516
  20. Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography.
    Biochem Biophys Res Commun. 1982 Jan 29;104(2):830-6 PMID: 6803791
  21. Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle.
    J Cell Biol. 1984 Sep;99(3):962-70 PMID: 6381510
  22. The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells.
    J Cell Biol. 1988 Aug;107(2):635-41 PMID: 3047144
  23. Troponin and parvalbumin calcium binding regions predicted in myosin light chain and T4 lysozyme.
    Science. 1975 Jan 17;187(4172):167-9 PMID: 1111094
  24. cDNA cloning and expression in Escherichia coli of a plasminogen activator inhibitor from human placenta.
    J Biol Chem. 1987 Mar 15;262(8):3718-25 PMID: 3029122
  25. Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences.
    J Mol Evol. 1990 Jun;30(6):522-62 PMID: 2115931
  26. Identification and localization of a novel, cytoskeletal, centrosome-associated protein in PtK2 cells.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2669-78 PMID: 3060471
  27. Calcium-modulated contractile proteins associated with the eucaryotic centrosome.
    Cell Motil Cytoskeleton. 1986;6(2):193-7 PMID: 3518957
  28. Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5512-6 PMID: 3526331
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11039
Published
1993-12-01
Pages
11039-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47917
Subset
IM
Grants
NIGMS NIH HHS · GM38113 · United States
Databases
GENBANK
X72964
External Links
PubMed source DOI: 10.1073/pnas.90.23.11039 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2