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PMID: 8257601 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Targeting and retention of Golgi membrane proteins.

Current opinion in cell biology ·Vol. 5 ·No. 4 ·1993-08-00 ·Pages 606-12

Machamer CE

Abstract

Recent cloning of genes encoding membrane proteins of the Golgi complex has allowed investigation of protein targeting to this organelle. Targeting signals have been identified in three glycosyltransferases, a viral envelope protein and several proteins of the trans-Golgi network. Interestingly, the targeting signals for membrane proteins of the Golgi stacks seem to be contained in transmembrane domains. Information in the cytoplasmic tails is required for the targeting of trans-Golgi network proteins. Mechanisms involving both retention and retrieval have been invoked.

MeSH Terms
Biological Transport/physiology Golgi Apparatus/chemistry,physiology Humans Membrane Proteins/metabolism Signal Transduction/physiology Structure-Activity Relationship
Chemicals
Membrane Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Machamer C E
Department of Cell Biology and Anatomy, Johns Hopkins Medical School, Baltimore, Maryland 21205.
References (49)
49 references, click to expand
  1. Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.
    J Biol Chem. 1991 Nov 15;266(32):21327-30 PMID: 1939168
  2. Retention of a cis Golgi protein requires polar residues on one face of a predicted alpha-helix in the transmembrane domain.
    Mol Biol Cell. 1993 Jul;4(7):695-704 PMID: 8400455
  3. Evidence for a molecular distinction between Golgi and cell surface forms of beta 1,4-galactosyltransferase.
    J Biol Chem. 1991 Aug 25;266(24):15984-91 PMID: 1714903
  4. Bidirectional membrane traffic between the endoplasmic reticulum and Golgi apparatus.
    Trends Cell Biol. 1993 Mar;3(3):81-8 PMID: 14731776
  5. Enzymes required for yeast prohormone processing.
    Annu Rev Physiol. 1988;50:345-62 PMID: 3288097
  6. A specific transmembrane domain of a coronavirus E1 glycoprotein is required for its retention in the Golgi region.
    J Cell Biol. 1987 Sep;105(3):1205-14 PMID: 2821010
  7. Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane.
    J Cell Biol. 1992 Dec;119(6):1459-68 PMID: 1469044
  8. Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment.
    J Cell Biol. 1992 Oct;119(1):69-83 PMID: 1527174
  9. Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence.
    J Cell Biol. 1993 Mar;120(5):1123-35 PMID: 8436587
  10. Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
    Annu Rev Biochem. 1987;56:829-52 PMID: 3304148
  11. Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans.
    J Cell Biol. 1991 Dec;115(6):1521-34 PMID: 1757461
  12. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
    Biochem J. 1990 Aug 15;270(1):97-102 PMID: 2204342
  13. Beta 1,4-galactosyltransferase: a short NH2-terminal fragment that includes the cytoplasmic and transmembrane domain is sufficient for Golgi retention.
    J Biol Chem. 1992 May 5;267(13):9241-7 PMID: 1374389
  14. Endocytosis and signals for internalization.
    Curr Opin Cell Biol. 1991 Aug;3(4):634-41 PMID: 1663371
  15. Mammalian subtilisins: the long-sought dibasic processing endoproteases.
    Cell. 1991 Jul 12;66(1):1-3 PMID: 2070411
  16. The signal for Golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain.
    J Biol Chem. 1992 Feb 25;267(6):4084-96 PMID: 1637374
  17. Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells.
    J Cell Biol. 1993 Jan;120(1):5-13 PMID: 8416995
  18. Sequences within and adjacent to the transmembrane segment of alpha-2,6-sialyltransferase specify Golgi retention.
    EMBO J. 1991 Dec;10(12):3577-88 PMID: 1935890
  19. The signal anchor and stem regions of the beta-galactoside alpha 2,6-sialyltransferase may each act to localize the enzyme to the Golgi apparatus.
    J Biol Chem. 1992 Apr 15;267(11):7784-93 PMID: 1560012
  20. The trans Golgi network: sorting at the exit site of the Golgi complex.
    Science. 1986 Oct 24;234(4775):438-43 PMID: 2945253
  21. Golgi retention of a trans-Golgi membrane protein, galactosyltransferase, requires cysteine and histidine residues within the membrane-anchoring domain.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4319-23 PMID: 1584766
  22. Pathways of protein sorting and membrane traffic between the rough endoplasmic reticulum and the Golgi complex.
    Semin Cell Biol. 1992 Oct;3(5):343-55 PMID: 1457777
  23. Recycling of proteins between the endoplasmic reticulum and Golgi complex.
    Curr Opin Cell Biol. 1991 Aug;3(4):585-91 PMID: 1663369
  24. Oligomerization of a membrane protein correlates with its retention in the Golgi complex.
    J Cell Biol. 1993 Sep;122(6):1185-96 PMID: 8397214
  25. Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole.
    Mol Biol Cell. 1992 Dec;3(12):1353-71 PMID: 1493334
  26. The 17-residue transmembrane domain of beta-galactoside alpha 2,6-sialyltransferase is sufficient for Golgi retention.
    J Cell Biol. 1992 Apr;117(2):245-58 PMID: 1560026
  27. Targeting of viral glycoproteins to the Golgi complex.
    Trends Microbiol. 1993 Jul;1(4):124-30 PMID: 8143127
  28. The membrane spanning domain of beta-1,4-galactosyltransferase specifies trans Golgi localization.
    EMBO J. 1991 Dec;10(12):3567-75 PMID: 1935889
  29. Translocation of nucleotide sugars and nucleotide sulphate across membranes of the endoplasmic reticulum and Golgi apparatus.
    Biochem Soc Trans. 1989 Jun;17(3):447-8 PMID: 2753215
  30. The Golgi complex: in vitro veritas?
    Cell. 1992 Mar 6;68(5):829-40 PMID: 1547485
  31. The transmembrane and flanking sequences of beta 1,2-N-acetylglucosaminyltransferase I specify medial-Golgi localization.
    J Biol Chem. 1992 Dec 5;267(34):24433-40 PMID: 1447191
  32. Compartmental organization of the Golgi stack.
    Cell. 1985 Aug;42(1):13-21 PMID: 3926324
  33. Two integral membrane proteins located in the cis-middle and trans-part of the Golgi system acquire sialylated N-linked carbohydrates and display different turnovers and sensitivity to cAMP-dependent phosphorylation.
    J Cell Biol. 1987 Jul;105(1):215-27 PMID: 3301866
  34. The E1 glycoprotein of an avian coronavirus is targeted to the cis Golgi complex.
    Proc Natl Acad Sci U S A. 1990 Sep;87(18):6944-8 PMID: 2169615
  35. The endoplasmic reticulum-Golgi intermediate compartment.
    Curr Opin Cell Biol. 1992 Aug;4(4):600-8 PMID: 1419041
  36. Lysosomal enzyme targeting.
    Biochem Soc Trans. 1990 Jun;18(3):367-74 PMID: 2164980
  37. Golgi retention signals: do membranes hold the key?
    Trends Cell Biol. 1991 Dec;1(6):141-4 PMID: 14731855
  38. Molecular dissection of the secretory pathway.
    Nature. 1992 Jan 30;355(6359):409-15 PMID: 1734280
  39. Characterization of two cis-regulatory regions in the murine beta 1,4-galactosyltransferase gene. Evidence for a negative regulatory element that controls initiation at the proximal site.
    J Biol Chem. 1993 Jul 5;268(19):14348-59 PMID: 8314795
  40. A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein.
    J Cell Biol. 1991 Oct;115(1):19-30 PMID: 1655802
  41. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  42. MG-160. A novel sialoglycoprotein of the medial cisternae of the Golgi apparatus [published eeratum appears in J Biol Chem 1989 Mar 5;264(7):4264].
    J Biol Chem. 1989 Jan 5;264(1):646-53 PMID: 2909545
  43. O-glycosylation of the coronavirus M protein. Differential localization of sialyltransferases in N- and O-linked glycosylation.
    J Biol Chem. 1992 Jul 15;267(20):14094-101 PMID: 1629209
  44. The Golgi sorting domain of coronavirus E1 protein.
    J Cell Sci. 1991 Apr;98 ( Pt 4):567-75 PMID: 1860903
  45. Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
    EMBO J. 1990 Oct;9(10):3153-62 PMID: 2120038
  46. The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal.
    J Biol Chem. 1992 May 15;267(14):10122-6 PMID: 1533634
  47. Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation.
    J Biol Chem. 1989 Oct 25;264(30):17615-8 PMID: 2681181
  48. Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae.
    J Cell Biol. 1992 Aug;118(3):531-40 PMID: 1322413
  49. Progress in unraveling pathways of Golgi traffic.
    Annu Rev Cell Biol. 1985;1:447-88 PMID: 3916320
Article Info
Journal
Current opinion in cell biology
Abbr.
Curr Opin Cell Biol
ISSN
0955-0674
DOI
10.1016/0955-0674(93)90129-e
Published
1993-08-00
Pages
606-12
Language
English
Region
England
NLM ID
8913428
PMCID
PMC7135176
Subset
IM
Grants
NIDDK NIH HHS · DK44375 · United States
NIGMS NIH HHS · GM42522 · United States
External Links
PubMed source DOI: 10.1016/0955-0674(93)90129-e PMC Full Text View journal details
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