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PMID: 8278379 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Autoregulation of I kappa B alpha activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 1 ·1994-01-04 ·Pages 28-32

Chiao PJ, Miyamoto S, Verma IM

Abstract

Transcription factor NF-kappa B regulates the expression of a plethora of genes. The activity of NF-kappa B proteins is regulated by I kappa B proteins. We report that induction of I kappa B alpha, a member of the I kappa B family of proteins, is preceded by activation of NF-kappa B complex. The promoter of the I kappa B alpha gene contains a kappa B site that is directly involved in its induction by the NF-kappa B complex. Degradation of I kappa B alpha protein precedes activation of NF-kappa B DNA binding activity, whereas newly synthesized I kappa B alpha protein inhibits NF-kappa B activity. If the degradation of I kappa B alpha is prevented, the induction of DNA binding activity of NF-kappa B complex is severely curtailed. These data suggest the existence of an autoregulatory loop whereby I kappa B alpha regulates the activity of transcription factor NF-kappa B, which in turn regulates the I kappa B alpha activity.

MeSH Terms
Animals Base Sequence Cell Line Cycloheximide/pharmacology Gene Expression Regulation/drug effects Mice Molecular Sequence Data NF-kappa B/antagonists & inhibitors,genetics Oligodeoxyribonucleotides/chemistry Promoter Regions, Genetic Proto-Oncogene Proteins/genetics RNA, Messenger/genetics Tosylphenylalanyl Chloromethyl Ketone/pharmacology Transcription Factor RelB Transcription Factors
Chemicals
NF-kappa B Oligodeoxyribonucleotides Proto-Oncogene Proteins RNA, Messenger Relb protein, mouse Transcription Factors Transcription Factor RelB Tosylphenylalanyl Chloromethyl Ketone Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chiao P J
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186-5800.
Miyamoto S
Verma I M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.1.28
Published
1994-01-04
Pages
28-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42879
Subset
IM
Grants
NCI NIH HHS · 1F32CA09115 · United States
NCI NIH HHS · 2T32CA09370 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.1.28 PMC Full Text View journal details
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