Home LiteratureArticle Details
PMID: 8285640 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reduced susceptibilities of Shigella sonnei strains isolated from patients with dysentery to fluoroquinolones.

Antimicrobial agents and chemotherapy ·Vol. 37 ·No. 11 ·1993-11-00 ·Pages 2486-9

Horiuchi S, Inagaki Y, Yamamoto N, Okamura N, Imagawa Y, Nakaya R

Abstract

Seven clinical isolates of Shigella sonnei with reduced susceptibilities to fluoroquinolones (sparfloxacin, ciprofloxacin, and ofloxacin) were obtained. The MICs of fluoroquinolones against these S. sonnei strains were 16 to 32 times higher than those obtained against typical strains that are highly susceptible to these agents. The kinetics of [14C]ofloxacin accumulation in these clinical strains were not different from those in the fully susceptible strains. However, DNA synthesis was much less inhibited by ofloxacin in the strains with reduced susceptibility. Analysis of the in vitro activity of the partially purified DNA gyrase from these isolates showed that the decreased quinolone susceptibility of the S. sonnei strains was likely due to mutation of the DNA gyrase subunit A gene.

MeSH Terms
Anti-Infective Agents/pharmacology DNA Topoisomerases, Type II/metabolism DNA, Bacterial/biosynthesis Drug Resistance, Microbial Dysentery, Bacillary/microbiology Fluoroquinolones Humans Microbial Sensitivity Tests Shigella sonnei/drug effects
Chemicals
Anti-Infective Agents DNA, Bacterial Fluoroquinolones DNA Topoisomerases, Type II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Horiuchi S
Department of Microbiology, Tokyo Medical and Dental University, Japan.
Inagaki Y
Yamamoto N
Okamura N
Imagawa Y
Nakaya R
References (25)
25 references, click to expand
  1. Mechanisms of quinolone resistance in Escherichia coli: characterization of nfxB and cfxB, two mutant resistance loci decreasing norfloxacin accumulation.
    Antimicrob Agents Chemother. 1989 Mar;33(3):283-90 PMID: 2658782
  2. Mechanism of quinolone inhibition of DNA gyrase. Appearance of unique norfloxacin binding sites in enzyme-DNA complexes.
    J Biol Chem. 1989 Feb 15;264(5):2973-8 PMID: 2536729
  3. In vitro activities of lomefloxacin and temafloxacin against pathogens causing diarrhea.
    Antimicrob Agents Chemother. 1989 Aug;33(8):1385-7 PMID: 2802563
  4. Mechanisms of quinolone resistance in a clinical isolate of Escherichia coli highly resistant to fluoroquinolones but susceptible to nalidixic acid.
    Antimicrob Agents Chemother. 1991 Mar;35(3):519-23 PMID: 2039202
  5. Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli.
    Antimicrob Agents Chemother. 1991 Aug;35(8):1647-50 PMID: 1656869
  6. Antimicrobial resistance of Shigella isolates in Bangladesh, 1983-1990: increasing frequency of strains multiply resistant to ampicillin, trimethoprim-sulfamethoxazole, and nalidixic acid.
    Clin Infect Dis. 1992 May;14(5):1055-60 PMID: 1600006
  7. Transduction of R factors by a Proteus mirabilis bacteriophage.
    J Bacteriol. 1971 Jun;106(3):773-83 PMID: 4104237
  8. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  9. DNA gyrase: subunit structure and ATPase activity of the purified enzyme.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5960-3 PMID: 153529
  10. The intrinsic ATPase of DNA gyrase.
    J Biol Chem. 1980 Jul 10;255(13):6299-306 PMID: 6248518
  11. In vitro and in vivo activity of DL-8280, a new oxazine derivative.
    Antimicrob Agents Chemother. 1982 Oct;22(4):548-53 PMID: 6960805
  12. DNA gyrase (Topoisomerase II) from Pseudomonas aeruginosa.
    Biochem Biophys Res Commun. 1983 Jan 27;110(2):694-700 PMID: 6301461
  13. Ciprofloxacin, a quinolone carboxylic acid compound active against aerobic and anaerobic bacteria.
    Antimicrob Agents Chemother. 1984 Mar;25(3):319-26 PMID: 6232895
  14. Clinical resistance to long-term oral ciprofloxacin.
    Lancet. 1985 Jun 1;1(8440):1275 PMID: 2860471
  15. The fluoroquinolones: structures, mechanisms of action and resistance, and spectra of activity in vitro.
    Antimicrob Agents Chemother. 1985 Oct;28(4):581-6 PMID: 3000292
  16. In-vitro activity of ofloxacin, a quinolone carboxylic acid compared to other quinolones and other antimicrobial agents.
    J Antimicrob Chemother. 1985 Nov;16(5):563-74 PMID: 3865923
  17. The fluoroquinolones: pharmacology, clinical uses, and toxicities in humans.
    Antimicrob Agents Chemother. 1985 Nov;28(5):716-21 PMID: 2936302
  18. Genetic and biochemical characterization of norfloxacin resistance in Escherichia coli.
    Antimicrob Agents Chemother. 1986 Apr;29(4):639-44 PMID: 3010850
  19. Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.
    Antimicrob Agents Chemother. 1986 Nov;30(5):777-80 PMID: 3026239
  20. Effect of new quinolones on the human gastrointestinal microflora.
    Rev Infect Dis. 1988 Jan-Feb;10 Suppl 1:S193-6 PMID: 3279492
  21. In vitro and in vivo antibacterial activities of T-3262, a new fluoroquinolone.
    Antimicrob Agents Chemother. 1988 Jun;32(6):827-33 PMID: 2843082
  22. Clinical isolate of Citrobacter freundii highly resistant to new quinolones.
    Antimicrob Agents Chemother. 1988 Jun;32(6):922-4 PMID: 2843087
  23. Association between early inhibition of DNA synthesis and the MICs and MBCs of carboxyquinolone antimicrobial agents for wild-type and mutant [gyrA nfxB(ompF) acrA] Escherichia coli K-12.
    Antimicrob Agents Chemother. 1988 Aug;32(8):1113-8 PMID: 3056251
  24. Development of resistance to nalidixic acid and the fluoroquinolones after the introduction of norfloxacin and ofloxacin.
    Antimicrob Agents Chemother. 1988 Aug;32(8):1285-8 PMID: 3142353
  25. Cross-resistance to fluoroquinolones in multiple-antibiotic-resistant (Mar) Escherichia coli selected by tetracycline or chloramphenicol: decreased drug accumulation associated with membrane changes in addition to OmpF reduction.
    Antimicrob Agents Chemother. 1989 Aug;33(8):1318-25 PMID: 2679373
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.37.11.2486
Published
1993-11-00
Pages
2486-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC192415
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.37.11.2486 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2