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PMID: 8336735 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns.

Molecular and cellular biology ·Vol. 13 ·No. 8 ·1993-08-00 ·Pages 5070-84

Bauer F, Urdaci M, Aigle M, Crouzet M

Abstract

Mutations in genes necessary for survival in stationary phase were isolated to understand the ability of wild-type Saccharomyces cerevisiae to remain viable during prolonged periods of nutritional deprivation. Here we report results concerning one of these mutants, rvs167, which shows reduced viability and abnormal cell morphology upon carbon and nitrogen starvation. The mutant exhibits the same response when cells are grown in high salt concentrations and other unfavorable growth conditions. The RVS167 gene product displays significant homology with the Rvs161 protein and contains a SH3 domain at the C-terminal end. Abnormal actin distribution is associated with the mutant phenotype. In addition, while the budding pattern of haploid strains remains axial in standard growth conditions, the budding pattern of diploid mutant strains is random. The gene RVS167 therefore could be implicated in cytoskeletal reorganization in response to environmental stresses and could act in the budding site selection mechanism.

Related Genes
ABP1 Fod MYO MyoA MyoD Plc RVS161 RVS167 c-abl c-src c-yes
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cytoskeleton/ultrastructure DNA Mutational Analysis Fungal Proteins/genetics,metabolism Gene Expression Genes, Fungal Microfilament Proteins Molecular Sequence Data Mutagenesis, Insertional RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins Sequence Alignment
Chemicals
Fungal Proteins Microfilament Proteins RNA, Messenger RVS167 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bauer F
Laboratoire de Génétique, Université de Bordeaux II, Talence, France.
Urdaci M
Aigle M
Crouzet M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.8.5070-5084.1993
Published
1993-08-00
Pages
5070-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360159
Subset
IM
Databases
GENBANK
M92092
External Links
PubMed source DOI: 10.1128/mcb.13.8.5070-5084.1993 PMC Full Text View journal details
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