Home LiteratureArticle Details
PMID: 8355697 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

MPTP delta, a putative murine homolog of HPTP delta, is expressed in specialized regions of the brain and in the B-cell lineage.

Molecular and cellular biology ·Vol. 13 ·No. 9 ·1993-09-00 ·Pages 5513-23

Mizuno K, Hasegawa K, Katagiri T, Ogimoto M, Ichikawa T, Yakura H

Abstract

Protein tyrosine phosphatases (PTPs), together with protein tyrosine kinases (PTKs), are involved in the regulation of cell activation, growth, and differentiation. To further elucidate the fine tuning of cell growth and differentiation through tyrosine phosphorylation, we tried to isolate mouse receptor-type PTP (RPTP) cDNA clones by screening mouse brain cDNA libraries with mouse CD45 PTP domain probes under reduced-stringency conditions. Characterization of isolated cDNA clones for RPTP showed that the cytoplasmic region contains two tandem repeats of PTP domain of about 230 amino acids with intrinsic phosphatase activity. The extracellular region was composed of immunoglobulin (Ig)-like domains and fibronectin type III (FN-III)-like domains. The gene was highly homologous to human PTP delta (HPTP delta) and thus was named MPTP delta (murine counterpart of HPTP delta). The MPTP delta gene appeared to generate at least three species of mRNA, which differ in the composition of the extracellular domain: type A, one Ig-like and four FN-III-like domains; type B, one Ig-like and eight FN-III-like domains; and type C, three Ig-like and eight FN-III-like domains. Interestingly, the 5' untranslated region and the leader peptide of types A and B were completely different from those of type C. Northern (RNA) blot analysis demonstrated that brain, kidney, and heart cells express three mRNA species of about 7 kb. Antibody directed against part of the extracellular domain of type A MPTP delta recognized a 210-kDa protein in brain and kidney lysates. In situ hybridization of brain samples revealed that MPTP delta mRNA is present in the hippocampus, thalamic reticular nucleus, and piriform cortex, where some Src family PTKs have been also demonstrated to exist. Although MPTP delta mRNA was not detected in lymphoid tissues, all of the pre-B-cell lines tested and one of three B-cell lines tested expressed MPTP delta mRNA, whereas antibody-producing B-cell hybridomas and T-cell and macrophage lines did not. Finally, the MPTP delta locus was tightly linked to the brown (b) locus on mouse chromosome 4.

Related Genes
CD45 FN-III HPTP&dgr LAR MPTP&dgr N-CAM
MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/enzymology Base Sequence Brain/enzymology Chromosome Mapping Cloning, Molecular Gene Expression In Situ Hybridization Mice Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Protein Tyrosine Phosphatases/genetics RNA, Messenger/genetics Recombinant Proteins/metabolism Restriction Mapping Sequence Alignment
Chemicals
Oligodeoxyribonucleotides RNA, Messenger Recombinant Proteins Protein Tyrosine Phosphatases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mizuno K
Tokyo Metropolitan Institute for Neuroscience, Japan.
Hasegawa K
Katagiri T
Ogimoto M
Ichikawa T
Yakura H
References (52)
52 references, click to expand
  1. The SJL/J T cell response to both spontaneous and transplantable syngeneic reticulum cell sarcoma is mediated predominantly by the V beta 17a+ T cell clonotype.
    J Exp Med. 1988 Nov 1;168(5):1553-62 PMID: 2460577
  2. Growth factor receptor tyrosine kinases.
    Annu Rev Biochem. 1988;57:443-78 PMID: 3052279
  3. Brain-specific src oncogene mRNA mapped in rat brain by in situ hybridization.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9831-5 PMID: 3200860
  4. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase.
    Biochemistry. 1988 Nov 29;27(24):8695-701 PMID: 2853967
  5. The leukocyte common antigen family.
    Annu Rev Immunol. 1989;7:339-69 PMID: 2523715
  6. A functional dichotomy in CD4+ T lymphocytes.
    Immunol Today. 1988 Sep;9(9):268-74 PMID: 2908229
  7. Phenotypic and functional heterogeneity of CD4+ T cells.
    Immunol Today. 1988 Sep;9(9):274-7 PMID: 2908230
  8. Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation.
    Cell. 1989 Sep 22;58(6):1055-65 PMID: 2550143
  9. A family of receptor-linked protein tyrosine phosphatases in humans and Drosophila.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8698-702 PMID: 2554325
  10. Expression of CD45 alters phosphorylation of the lck-encoded tyrosine protein kinase in murine lymphoma T-cell lines.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8959-63 PMID: 2530588
  11. Allelic losses of chromosomes 9, 11, and 17 in human bladder cancer.
    Cancer Res. 1990 Jan 1;50(1):44-7 PMID: 2293558
  12. In situ localization of CD45 isoforms in the human thymus indicates a medullary location for the thymic generative lineage.
    J Immunol. 1990 Jan 1;144(1):66-74 PMID: 1688577
  13. The current excitement in long-term potentiation.
    Neuron. 1988 Apr;1(2):97-103 PMID: 2856092
  14. Signal transduction by receptors with tyrosine kinase activity.
    Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
  15. Genetic analysis of resistance to radiation lymphomagenesis with recombinant inbred strains of mice.
    Cancer Res. 1990 Jul 1;50(13):3848-50 PMID: 2354437
  16. Dephosphorylation and activation of the T cell tyrosine kinase pp56lck by the leukocyte common antigen (CD45).
    Oncogene. 1990 Jun;5(6):809-13 PMID: 2141684
  17. Tyrosine phosphatase CD45 is essential for coupling T-cell antigen receptor to the phosphatidyl inositol pathway.
    Nature. 1990 Jul 5;346(6279):66-8 PMID: 2164155
  18. Immunocytochemical localization of the neuron-specific form of the c-src gene product, pp60c-src(+), in rat brain.
    J Neurosci. 1990 Aug;10(8):2513-27 PMID: 1696980
  19. Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell-mediated chromosome transfer.
    Oncogene. 1990 Aug;5(8):1141-7 PMID: 2392323
  20. Allelic loss of chromosome 17p distinguishes high grade from low grade transitional cell carcinomas of the bladder.
    Cancer Res. 1990 Nov 1;50(21):7081-3 PMID: 2208176
  21. Structural diversity and evolution of human receptor-like protein tyrosine phosphatases.
    EMBO J. 1990 Oct;9(10):3241-52 PMID: 2170109
  22. Inhibition of tyrosine phosphorylation prevents T-cell receptor-mediated signal transduction.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7722-6 PMID: 2217205
  23. The role of phosphatases in signal transduction.
    New Biol. 1990 Dec;2(12):1049-62 PMID: 1965146
  24. Receptor protein-tyrosine phosphatase gamma is a candidate tumor suppressor gene at human chromosome region 3p21.
    Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):5036-40 PMID: 1711217
  25. Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes.
    Science. 1991 Jul 26;253(5018):401-6 PMID: 1650499
  26. Cloning, expression and chromosomal localization of a new putative receptor-like protein tyrosine phosphatase.
    FEBS Lett. 1991 Sep 23;290(1-2):123-30 PMID: 1655529
  27. Long-term potentiation in the hippocampus is blocked by tyrosine kinase inhibitors.
    Nature. 1991 Oct 10;353(6344):558-60 PMID: 1656271
  28. CD45: a leukocyte-specific member of the protein tyrosine phosphatase family.
    Biochim Biophys Acta. 1991 Oct 16;1095(1):46-56 PMID: 1834176
  29. Expression of the receptor-linked protein tyrosine phosphatase LAR: proteolytic cleavage and shedding of the CAM-like extracellular region.
    EMBO J. 1992 Mar;11(3):897-907 PMID: 1547787
  30. Regulation of lipopolysaccharide- and IL-4-induced immunoglobulin heavy chain gene activation: differential roles for CD45 and Lyb-2.
    Int Immunol. 1992 Jun;4(6):651-9 PMID: 1535509
  31. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  32. On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.
    J Exp Med. 1983 Apr 1;157(4):1077-88 PMID: 6220106
  33. Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins.
    Cell. 1985 Jul;41(3):707-17 PMID: 2408760
  34. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
  35. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene.
    EMBO J. 1985 Jul;4(7):1755-9 PMID: 2992939
  36. Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody.
    J Immunol. 1986 Apr 15;136(8):2729-33 PMID: 3485673
  37. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  38. Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6940-4 PMID: 2944116
  39. Rearrangement of genes located on homologous chromosomal segments in mouse and man: the location of genes for alpha- and beta-interferon, alpha-1 acid glycoprotein-1 and -2, and aminolevulinate dehydratase on mouse chromosome 4.
    Genetics. 1986 Dec;114(4):1239-55 PMID: 3100390
  40. A tail of two src's: mutatis mutandis.
    Cell. 1987 Apr 10;49(1):1-4 PMID: 3030562
  41. Isolation and nucleotide sequence of mouse NCAM cDNA that codes for a Mr 79,000 polypeptide without a membrane-spanning region.
    EMBO J. 1987 Apr;6(4):907-14 PMID: 3595563
  42. B-cell variant of mouse T200 (Ly-5): evidence for alternative mRNA splicing.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5360-3 PMID: 2955416
  43. Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5364-8 PMID: 3037546
  44. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  45. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  46. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.
    J Exp Med. 1987 Nov 1;166(5):1548-66 PMID: 2824653
  47. Experimental models for understanding B lymphocyte formation.
    Adv Immunol. 1987;41:181-267 PMID: 3318292
  48. Cell transformation by the viral src oncogene.
    Annu Rev Cell Biol. 1987;3:31-56 PMID: 2446642
  49. Alternative promoters in developmental gene expression.
    Annu Rev Genet. 1987;21:237-57 PMID: 2450521
  50. Poly-N-acetyllactosamine structures on murine cell surface T200 glycoprotein participate in natural killer cell binding to YAC-1 targets.
    J Immunol. 1988 Apr 15;140(8):2821-8 PMID: 2965731
  51. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7182-6 PMID: 2845400
  52. CD45 regulates signal transduction and lymphocyte activation by specific association with receptor molecules on T or B cells.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8628-32 PMID: 2973067
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.9.5513-5523.1993
Published
1993-09-00
Pages
5513-23
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360267
Subset
IM
Databases
GENBANK
D13903, D13904, D13905
External Links
PubMed source DOI: 10.1128/mcb.13.9.5513-5523.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2