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PMID: 8382611 Published · ppublish English Journal Article

Cell surface control of the multiubiquitination and deubiquitination of high-affinity immunoglobulin E receptors.

The EMBO journal ·Vol. 12 ·No. 2 ·1993-02-00 ·Pages 779-86

Paolini R, Kinet JP

Abstract

Multiubiquitination of proteins is a critical step leading to selective degradation for many polypeptides. Therefore, activation-induced multiubiquitination of cell surface receptors, such as the platelet-derived growth factor (PDGF) receptor and the T cell antigen (TCR) receptor, may correspond to a degradation pathway for ligand-receptor complexes. Here we show that the antigen-induced engagement of high-affinity immunoglobulin E receptors (Fc epsilon RI) results in the immediate multiubiquitination of Fc epsilon RI beta and gamma chains. This ubiquitination is independent of receptor phosphorylation and is restricted to activated receptors. Surprisingly, receptor multiubiquitination is immediately reversible when receptors are disengaged. Therefore, multiubiquitination and deubiquitination of Fc epsilon RI receptors is controlled at the cell surface by receptor engagement and disengagement. The rapidity, specificity and, most importantly, the reversibility of the activation-induced receptor multiubiquitination suggest that this process may turn on/off a cell surface receptor signaling function thus far unsuspected.

MeSH Terms
Animals Antigens/immunology Cell Membrane/metabolism Phosphoproteins/metabolism Phosphorylation Rats Receptors, IgE/metabolism Substrate Specificity Time Factors Tumor Cells, Cultured Ubiquitins/metabolism
Chemicals
Antigens Phosphoproteins Receptors, IgE Ubiquitins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Paolini R
Molecular Allergy and Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852.
Kinet J P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-02-00
Pages
779-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413265
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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