Home LiteratureArticle Details
PMID: 8384306 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The murine myeloperoxidase promoter contains several functional elements, one of which binds a cell type-restricted transcription factor, myeloid nuclear factor 1 (MyNF1).

Molecular and cellular biology ·Vol. 13 ·No. 4 ·1993-04-00 ·Pages 2141-51

Suzow J, Friedman AD

Abstract

The myeloperoxidase (MPO) gene is expressed specifically in myeloid cells. There is significant homology between the murine and human MPO genes in the 1.6-kb region located upstream of the murine MPO transcription initiation sites. 5',3', and internal deletions of this DNA segment localized several cis-acting DNA elements in the murine MPO promoter which are functional in 32D cl3 cells, a murine myeloblast cell line which expresses MPO. These DNA elements did not function well in mouse L-cell fibroblasts. Additional mutagenesis of the most active promoter region allowed the delimitation of a functional 20-bp segment. Mutation of the enhancer core motif within this segment was functionally deleterious, and an oligonucleotide containing these base pairs increased the activity of a minimal promoter. This same oligonucleotide, but not a mutant variant, could bind a set of nuclear proteins, myeloid nuclear factors 1 alpha and 1 beta (MyNF1 alpha and -1 beta), present in 32D cl3 cells but absent from L cells, murine erythroleukemia cells, and SP2 lymphoid cells.

MeSH Terms
Animals Base Sequence Cell Line DNA Mutational Analysis DNA-Binding Proteins/metabolism Gene Expression Regulation, Enzymologic Hematopoietic Stem Cells/physiology Humans Mice Molecular Sequence Data Nuclear Proteins/genetics,metabolism Oligodeoxyribonucleotides/chemistry Peroxidase/genetics Promoter Regions, Genetic RNA, Messenger/genetics Sequence Deletion Transcription Factors/genetics,metabolism
Chemicals
DNA-Binding Proteins Mynf1 protein, mouse Nuclear Proteins Oligodeoxyribonucleotides RNA, Messenger Transcription Factors Peroxidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suzow J
Division of Pediatric Oncology, Johns Hopkins Oncology Center, Johns Hopkins University, Baltimore, Maryland 21287.
Friedman A D
References (66)
66 references, click to expand
  1. A major positive regulatory region located far upstream of the human alpha-globin gene locus.
    Genes Dev. 1990 Sep;4(9):1588-601 PMID: 2253879
  2. Chromatin structural analysis of the 5' end and contiguous flanking region of the myeloperoxidase gene.
    Blood. 1991 Jan 1;77(1):159-64 PMID: 1845843
  3. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 PMID: 5277089
  4. A better cell line for making hybridomas secreting specific antibodies.
    Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
  5. Immunocytochemical localization of myeloperoxidase, lactoferrin, lysozyme and neutral proteases in human monocytes and neutrophilic granulocytes.
    J Reticuloendothel Soc. 1978 Sep;24(3):295-310 PMID: 83363
  6. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
    Cell. 1981 Aug;25(2):385-98 PMID: 6269744
  7. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  8. Chromatin changes accompany immunoglobulin kappa gene activation: a potential control region within the gene.
    Nature. 1982 Sep 30;299(5882):449-51 PMID: 6811947
  9. Multiple point mutations affecting the simian virus 40 enhancer.
    Science. 1983 Feb 11;219(4585):626-31 PMID: 6297005
  10. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  11. Single cell origin of multilineage colonies in culture. Evidence that differentiation of multipotent progenitors and restriction of proliferative potential of monopotent progenitors are stochastic processes.
    J Clin Invest. 1984 Dec;74(6):2193-7 PMID: 6511923
  12. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements.
    Cell. 1985 Jul;41(3):885-97 PMID: 3924411
  13. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  14. In vitro mutagenesis.
    Annu Rev Genet. 1985;19:423-62 PMID: 3002241
  15. Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
    Cell. 1986 Feb 28;44(4):565-76 PMID: 3004739
  16. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  17. Characterization of cDNA clones for human myeloperoxidase: predicted amino acid sequence and evidence for multiple mRNA species.
    Nucleic Acids Res. 1987 Mar 11;15(5):2013-28 PMID: 3031585
  18. Cytokine-dependent granulocytic differentiation. Regulation of proliferative and differentiative responses in a murine progenitor cell line.
    J Immunol. 1987 Jun 1;138(11):3829-35 PMID: 2438328
  19. The human hematopoietic colony-stimulating factors.
    Science. 1987 Jun 5;236(4806):1229-37 PMID: 3296190
  20. Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells.
    Mol Cell Biol. 1987 Jun;7(6):2267-75 PMID: 3496523
  21. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  22. Chromosomal gene structure of human myeloperoxidase and regulation of its expression by granulocyte colony-stimulating factor.
    J Biol Chem. 1987 Nov 5;262(31):15208-13 PMID: 2444596
  23. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses.
    Genes Dev. 1987 Apr;1(2):133-46 PMID: 2824279
  24. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
    Cell. 1988 Feb 12;52(3):415-23 PMID: 2964277
  25. Transcriptional regulation of myeloperoxidase gene expression in myeloid leukemia HL-60 cells during differentiation into granulocytes and macrophages.
    Arch Biochem Biophys. 1988 May 1;262(2):599-604 PMID: 2835011
  26. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
    EMBO J. 1988 Feb;7(2):377-84 PMID: 2835225
  27. Type 1 plasminogen activator inhibitor gene: functional analysis and glucocorticoid regulation of its promoter.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5525-9 PMID: 2840665
  28. Regulation of gene expression of myeloperoxidase during myeloid differentiation.
    J Cell Physiol. 1988 Aug;136(2):215-25 PMID: 2842344
  29. The HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence.
    Cell. 1988 Sep 23;54(7):931-42 PMID: 2843293
  30. A simple phase-extraction assay for chloramphenicol acyltransferase activity.
    Gene. 1988 Jul 30;67(2):271-7 PMID: 3169576
  31. The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.
    Genes Dev. 1988 Sep;2(9):1089-100 PMID: 2461328
  32. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations.
    Cell. 1991 Jun 28;65(7):1143-52 PMID: 1648448
  33. Changes of DNA methylation and chromatin structure in the human myeloperoxidase gene during myeloid differentiation.
    Blood. 1991 Jul 15;78(2):345-56 PMID: 1648980
  34. A retinoic acid-responsive human zinc finger gene, MZF-1, preferentially expressed in myeloid cells.
    J Biol Chem. 1991 Aug 5;266(22):14183-7 PMID: 1860835
  35. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.
    Genes Dev. 1991 Sep;5(9):1538-52 PMID: 1840554
  36. CCAAT displacement protein as a repressor of the myelomonocytic-specific gp91-phox gene promoter.
    J Biol Chem. 1991 Sep 5;266(25):16736-44 PMID: 1885602
  37. A novel lineage-specific nuclear factor regulates mb-1 gene transcription at the early stages of B cell differentiation.
    EMBO J. 1991 Nov;10(11):3409-17 PMID: 1915300
  38. Transcriptional regulation of two myeloid-specific genes, myeloperoxidase and lactoferrin, during differentiation of the murine cell line 32D C13.
    Blood. 1991 Nov 1;78(9):2426-32 PMID: 1718497
  39. The promoter of the CD11b gene directs myeloid-specific and developmentally regulated expression.
    Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10525-9 PMID: 1683702
  40. Characterization of the myeloid-specific CD11b promoter.
    Blood. 1992 Feb 15;79(4):865-70 PMID: 1346576
  41. Coordination of immunoglobulin DJH transcription and D-to-JH rearrangement by promoter-enhancer approximation.
    Mol Cell Biol. 1991 Apr;11(4):2096-107 PMID: 1900920
  42. A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer.
    Genes Dev. 1991 Apr;5(4):656-69 PMID: 2010090
  43. A transplantable myelomonocytic leukemia in BALB-c mice: cytology, karyotype, and muramidase content.
    J Natl Cancer Inst. 1969 Oct;43(4):963-82 PMID: 5259325
  44. TATA-binding protein is a classless factor.
    Cell. 1992 Mar 6;68(5):819-21 PMID: 1547484
  45. Promoter specificity of basal transcription factors.
    Cell. 1992 Mar 20;68(6):1135-44 PMID: 1547507
  46. Identification of the promoter of the myelomonocytic leukocyte integrin CD11b.
    Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2105-9 PMID: 1347945
  47. Inhibition of myeloid differentiation by the helix-loop-helix protein Id.
    Science. 1992 Mar 27;255(5052):1700-2 PMID: 1372755
  48. Regulatory elements that control the lineage-specific expression of myoD.
    Science. 1992 Apr 24;256(5056):538-42 PMID: 1315077
  49. Cloning and analysis of the CD18 promoter.
    Blood. 1992 May 15;79(10):2598-604 PMID: 1350225
  50. Hematopoietic transcription regulators and the origins of leukemia.
    Crit Rev Oncol Hematol. 1992 Mar;12(2):167-90 PMID: 1590941
  51. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor.
    Cell. 1992 Aug 21;70(4):585-93 PMID: 1505025
  52. Transcriptional regulation by triiodothyronine requires synergistic action of the thyroid receptor with another trans-acting factor.
    Mol Cell Biol. 1992 Sep;12(9):3991-7 PMID: 1324411
  53. A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells.
    Blood. 1992 Oct 1;80(7):1725-35 PMID: 1391942
  54. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
    Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
  55. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
    Nature. 1988 Dec 8;336(6199):551-7 PMID: 2904654
  56. A small-scale procedure for preparation of nuclear extracts that support efficient transcription and pre-mRNA splicing.
    Gene Anal Tech. 1988 Mar-Apr;5(2):22-31 PMID: 3192155
  57. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  58. Transcription factors AP-3 and AP-2 interact with the SV40 enhancer in a mutually exclusive manner.
    EMBO J. 1989 May;8(5):1455-60 PMID: 2548845
  59. The erythroid-specific transcription factor Eryf1: a new finger protein.
    Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
  60. Initiation of transcription by RNA polymerase II.
    Biochim Biophys Acta. 1989 Sep 21;1009(1):1-10 PMID: 2675975
  61. Complete nucleotide sequence of the human myeloperoxidase gene.
    Nucleic Acids Res. 1989 Oct 11;17(19):7985-6 PMID: 2552418
  62. Sequence of the murine myeloperoxidase (MPO) gene.
    Nucleic Acids Res. 1989 Oct 11;17(19):7987-8 PMID: 2552419
  63. The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene.
    Cell. 1989 Dec 22;59(6):1115-25 PMID: 2688896
  64. CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells.
    Genes Dev. 1989 Sep;3(9):1314-22 PMID: 2558052
  65. Detailed analysis of the site 3 region of the human beta-globin dominant control region.
    EMBO J. 1990 Jul;9(7):2169-77 PMID: 2357965
  66. Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium.
    Cell. 1990 Oct 5;63(1):195-201 PMID: 2208278
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.4.2141-2151.1993
Published
1993-04-00
Pages
2141-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359535
Subset
IM
Grants
NCI NIH HHS · CA01326 · United States
Databases
GENBANK
X15378
External Links
PubMed source DOI: 10.1128/mcb.13.4.2141-2151.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2