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PMID: 8404842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Olive: a key gene required for chlorophyll biosynthesis in Antirrhinum majus.

The EMBO journal ·Vol. 12 ·No. 10 ·1993-10-00 ·Pages 3711-9

Hudson A, Carpenter R, Doyle S, Coen ES

Abstract

Olive (oli) is a recessive nuclear mutation of Antirrhinum majus which reduces the level of chlorophyll pigmentation and affects the ultrastructure of chloroplasts. The oli-605 allele carries a Tam3 transposon insertion which has allowed the locus to be isolated. The oli gene encodes a large putative protein of 153 kDa which shows homology to the products of two bacterial genes necessary for tetrapyrrole-metal chelation during the synthesis of bacteriochlorophyll or cobyrinic acid. We therefore propose that the product of the oli gene is necessary for a key step of chlorophyll synthesis: the chelation of magnesium by protoporphyrin IX. Somatic reversion of the oli-605 allele produces chimeric plants which indicate that the oli gene functions cell-autonomously. Expression of oli is restricted to photosynthetic cells and repressed by light, suggesting that it may be involved in regulating the rate of chlorophyll synthesis in green tissues.

Related Genes
oli
MeSH Terms
Amino Acid Sequence Blotting, Northern Chlorophyll/biosynthesis,genetics Chloroplasts Cloning, Molecular Genes, Plant Molecular Sequence Data Molecular Structure Mutagenesis, Insertional Phenotype Plants/genetics Restriction Mapping Sequence Homology, Amino Acid
Chemicals
Chlorophyll
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hudson A
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Carpenter R
Doyle S
Coen E S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-10-00
Pages
3711-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413652
Subset
IM
Databases
GENBANK
X73144
External Links
PubMed source PMC Full Text View journal details
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