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PMID: 8413227 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Connections between the Ras-cyclic AMP pathway and G1 cyclin expression in the budding yeast Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 6274-82

Hubler L, Bradshaw-Rouse J, Heideman W

Abstract

We have identified two processes in the G1 phase of the Saccharomyces cerevisiae cell cycle that are required before nutritionally arrested cells are able to return to proliferative growth. The first process requires protein synthesis and is associated with increased expression of the G1 cyclin gene CLN3. This process requires nutrients but is independent of Ras and cyclic AMP (cAMP). The second process requires cAMP. This second process is rapid, is independent of protein synthesis, and produces a rapid induction of START-specific transcripts, including CLN1 and CLN2. The ability of a nutritionally arrested cell to respond to cAMP is dependent on completion of the first process, and this is delayed in cells carrying a CLN3 deletion. Mating pheromone blocks the cAMP response but does not alter the process upstream of Ras-cAMP. These results suggest a model linking the Ras-cAMP pathway with regulation of G1 cyclin expression.

Related Genes
CLN3
MeSH Terms
Blotting, Western Cyclic AMP/biosynthesis,genetics Cyclins/biosynthesis,genetics Fungal Proteins/biosynthesis,genetics G1 Phase Gene Expression Regulation, Fungal Genes, Fungal Genes, ras RNA, Fungal/metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
CLN3 protein, S cerevisiae Cyclins Fungal Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hubler L
School of Pharmacy, University of Wisconsin, Madison 53706.
Bradshaw-Rouse J
Heideman W
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.6274-6282.1993
Published
1993-10-00
Pages
6274-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364686
Subset
IM
Grants
NIGMS NIH HHS · GM42406 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.10.6274-6282.1993 PMC Full Text View journal details
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