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PMID: 8413250 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA bending by retinoid X receptor-containing retinoid and thyroid hormone receptor complexes.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 6509-19

Lu XP, Eberhardt NL, Pfahl M

Abstract

Retinoid X receptors (RXR) have been identified as common subunits in the regulation of multiple hormonal signaling pathways. Using circular permutation and phasing analysis of specific response elements, we present evidence that RXR-retinoic acid receptor and RXR-thyroid hormone receptor heterodimer or RXR-RXR homodimer complexes induce directed DNA bends when bound to their cognate response elements. The extent of DNA bending induced by the RXR alpha-containing complexes varied and depended on the structure of the DNA-binding sites and the RXR partners. The overall bending orientation for RXR-containing complexes is directed toward the major groove of the DNA helix at the center of hormone response elements. Our observation implicates DNA bending as a possible mechanism underlying transcriptional regulation of distinct retinoid and thyroid hormone responsive genes.

MeSH Terms
Base Sequence DNA/chemistry,metabolism Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Nucleic Acid Conformation Plasmids Protein Binding Receptors, Cytoplasmic and Nuclear/metabolism Receptors, Retinoic Acid Receptors, Thyroid Hormone/metabolism Retinoid X Receptors Retinoids/metabolism Transcription Factors
Chemicals
Receptors, Cytoplasmic and Nuclear Receptors, Retinoic Acid Receptors, Thyroid Hormone Retinoid X Receptors Retinoids Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lu X P
La Jolla Cancer Research Foundation, La Jolla, California 92037.
Eberhardt N L
Pfahl M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.6509-6519.1993
Published
1993-10-00
Pages
6509-19
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364710
Subset
IM
Grants
NCI NIH HHS · CA50676 · United States
NIDDK NIH HHS · DK41206 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.10.6509-6519.1993 PMC Full Text View journal details
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