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PMID: 8417352 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 561-70

Chatton B, Bocco JL, Gaire M, Hauss C, Reimund B, Goetz J, Kedinger C

Abstract

We recently isolated three cDNA clones encoding closely related proteins (ATFa1, ATFa2, and ATFa3) that belong to the activating transcription factor-cyclic AMP-responsive element family of cellular transcription factors. Using cotransfection experiments, we showed that these proteins mediate the transcriptional activation induced by the adenovirus E1a 13S mRNA gene product and that the zinc-binding domains present in both E1a conserved region 3 and the most N-terminal portion of the ATFa proteins play crucial roles in this activity. Reciprocal coimmunoprecipitation experiments demonstrated direct interactions between these proteins. Neither the conserved region 3 domain of E1a nor the N-terminal metal-binding element of ATFa is essential for these interactions. The simultaneous alteration of both the N-terminal and the C-terminal domains of ATFa abolished E1a binding, while either mutation alone failed to impair these interactions.

MeSH Terms
Activating Transcription Factors Adenovirus E1A Proteins/physiology Adenoviruses, Human/genetics Amino Acid Sequence Base Sequence Blood Proteins/physiology DNA/genetics Gene Expression Regulation Humans Molecular Sequence Data Promoter Regions, Genetic Structure-Activity Relationship Transcription Factors/physiology Transcriptional Activation Zinc Fingers
Chemicals
Activating Transcription Factors Adenovirus E1A Proteins Blood Proteins Transcription Factors DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chatton B
Laboratoire de Génétique Moléculaire des Eucaryotes, Unité 184 de Biologie Moléculaire et de Génie Génétique, Institut National de la Santé et de la Recherche Médicale, Strasbourg, France.
Bocco J L
Gaire M
Hauss C
Reimund B
Goetz J
Kedinger C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.561-570.1993
Published
1993-01-00
Pages
561-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358935
Subset
IM
Databases
GENBANK
X57197
External Links
PubMed source DOI: 10.1128/mcb.13.1.561-570.1993 PMC Full Text 免费全文 View journal details
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