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PMID: 8432872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, induces protective immunity in a guinea pig model of Legionnaires' disease.

The Journal of clinical investigation ·Vol. 91 ·No. 2 ·1993-02-00 ·Pages 717-23

Blander SJ, Horwitz MA

Abstract

We have examined the capacity of the major cytoplasmic membrane protein (MCMP) of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, to induce protective immunity in a guinea pig model of Legionnaires' disease. We purified MCMP to homogeneity from L. pneumophila by buffer extraction, ion-exchange chromatography, and molecular sieve chromatography. Guinea pigs immunized with MCMP developed a strong cell-mediated immune response to the immunogen manifest by marked cutaneous delayed-type hypersensitivity. Guinea pigs immunized with MCMP and then challenged with a lethal aerosol dose of L. pneumophila exhibited a high level of protective immunity. Altogether, in four independent experiments, 55 of 64 (86%) animals immunized three times with 0.6-40 micrograms MCMP including 11 of 11 (100%) animals immunized three times with 40 micrograms MCMP survived aerosol challenge with L. pneumophila compared with 1 of 29 (3%) sham-immunized control animals (P < 0.0001, Cochran-Mantel-Haenszel X2 statistic for pooled data). To our knowledge, MCMP is the first member of the hsp 60 family of proteins shown to induce protective immunity to a microbial pathogen. MCMP has potential as a vaccine against Legionnaires' disease. Since MCMP is a genus common antigen, vaccination with a combination of MCMPs derived from different Legionella species has the potential of inducing protective immunity against all the major Legionella species causing human disease.

MeSH Terms
Animals Antigens, Bacterial/immunology Bacterial Proteins/immunology Guinea Pigs Heat-Shock Proteins/immunology Immunity, Cellular Immunization Legionella pneumophila/immunology Legionnaires' Disease/immunology Male Membrane Proteins/immunology,isolation & purification
Chemicals
Antigens, Bacterial Bacterial Proteins Heat-Shock Proteins Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blander S J
Department of Medicine, University of California, Los Angeles School of Medicine 90024.
Horwitz M A
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI116253
Published
1993-02-00
Pages
717-23
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC288014
Subset
IM
Grants
NIAID NIH HHS · AI-07126 · United States
NIAID NIH HHS · AI-22421 · United States
External Links
PubMed source DOI: 10.1172/JCI116253 PMC Full Text View journal details
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