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PMID: 8436264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effects of central asymmetry on the propagation of palindromic DNA in bacteriophage lambda are consistent with cruciform extrusion in vivo.

Genetics ·Vol. 133 ·No. 2 ·1993-02-00 ·Pages 143-8

Chalker AF, Okely EA, Davison A, Leach DR

Abstract

The propagation of lambda phages carrying long perfect palindromes has been compared with that of phages carrying imperfect palindromes with small regions of central asymmetry. The perfect palindromes confer a more deleterious phenotype than those with central asymmetry and the severity of the phenotype declines with the length of asymmetry in the range from O to 27 base pairs. These results argue that a center-dependent reaction is involved in the phenotypic effects of palindromic DNA sequences, consistent with the idea that cruciform extrusion occurs in vivo.

Related Genes
gam recBC recC red sbcB sbcC sbcD
MeSH Terms
Bacteriophage lambda/genetics,physiology Base Sequence DNA Replication DNA, Recombinant/genetics DNA, Superhelical/genetics Escherichia coli/genetics Molecular Sequence Data Nucleic Acid Conformation Phenotype Virus Replication
Chemicals
DNA, Recombinant DNA, Superhelical
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chalker A F
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
Okely E A
Davison A
Leach D R
References (28)
28 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/133.2.143
Published
1993-02-00
Pages
143-8
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205305
Subset
IM
Databases
GENBANK
L24529, X70503, X70523, X70524, X70525, X70526, X70527, X70528, Z15047, Z15048
External Links
PubMed source DOI: 10.1093/genetics/133.2.143 PMC Full Text View journal details
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