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PMID: 8468302 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of gonococcal transferrin-binding protein 1 causes Escherichia coli to bind human transferrin.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2448-50

Cornelissen CN, Biswas GD, Sparling PF

Abstract

The gene for gonococcal transferrin-binding protein 1 (TBP1) was cloned behind an inducible promoter in Escherichia coli. The resultant strain was capable of binding human transferrin with the same specificity as that of the gonococcus. E. coli expressing TBP1 did not internalize transferrin-bound iron or grow on transferrin as a sole iron source.

Related Genes

tbpA

MeSH Terms

Animals Bacterial Proteins/genetics Carrier Proteins/genetics,physiology Cattle Cloning, Molecular Escherichia coli/genetics,metabolism Horses Humans Iron-Binding Proteins Mice Neisseria gonorrhoeae/genetics Rats Sheep Species Specificity Transferrin/metabolism Transferrin-Binding Proteins

Chemicals

Bacterial Proteins Carrier Proteins Iron-Binding Proteins Transferrin Transferrin-Binding Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cornelissen C N

Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill 27599.

Biswas G D

Sparling P F

References (13)

13 references, click to expand

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Molecular mechanism of regulation of siderophore-mediated iron assimilation.
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Identification and characterization of the transferrin receptor from Neisseria meningitidis.
Mol Microbiol. 1988 Mar;2(2):281-8 PMID: 3132585
Loss of transferrin receptor activity in Neisseria meningitidis correlates with inability to use transferrin as an iron source.
Infect Immun. 1988 Dec;56(12):3132-8 PMID: 3141281
Specificity of the lactoferrin and transferrin receptors in Neisseria gonorrhoeae.
Mol Microbiol. 1988 Nov;2(6):827-9 PMID: 2850444
Comparative analysis of the transferrin and lactoferrin binding proteins in the family Neisseriaceae.
Can J Microbiol. 1989 Mar;35(3):409-15 PMID: 2543489
Use of T7 RNA polymerase to direct expression of cloned genes.
Methods Enzymol. 1990;185:60-89 PMID: 2199796
Genetic evidence that Neisseria gonorrhoeae produces specific receptors for transferrin and lactoferrin.
J Bacteriol. 1990 Sep;172(9):5225-35 PMID: 2168377
Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence.
J Bacteriol. 1991 Jun;173(12):3911-3 PMID: 1904861
Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors.
J Bacteriol. 1992 Sep;174(18):5788-97 PMID: 1325963
Formation of a gated channel by a ligand-specific transport protein in the bacterial outer membrane.
Science. 1992 Oct 16;258(5081):471-5 PMID: 1411544
NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.175.8.2448-2450.1993

Published

1993-04-00

Pages

2448-50

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC204535

Subset

Grants

NIAID NIH HHS · AI26837 · United States

NIAID NIH HHS · AI31496 · United States

External Links

PubMed source DOI: 10.1128/jb.175.8.2448-2450.1993 PMC Full Text View journal details

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Expression of gonococcal transferrin-binding protein 1 causes Escherichia coli to bind human transferrin.

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