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PMID: 8514394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The RTX cytotoxin-related FrpA protein of Neisseria meningitidis is secreted extracellularly by meningococci and by HlyBD+ Escherichia coli.

Infection and immunity ·Vol. 61 ·No. 7 ·1993-07-00 ·Pages 2906-11

Thompson SA, Sparling PF

Abstract

Neisseria meningitidis produces proteins (FrpA and FrpC) related to the RTX cytotoxin family. In meningococcal strain FAM20 these proteins were both localized in the outer membrane and secreted into the extracellular medium. An Escherichia coli strain with wild-type hemolysin secretion genes hlyB and hlyD and containing a cloned frpA gene secreted FrpA, whereas an isogenic hlyBD mutant strain did not. Low-stringency DNA hybridization revealed hlyBD-like sequences in N. meningitidis FAM20, suggesting that a similar RTX secretion system exists in meningococci. Structural features found at the C termini of other RTX proteins and thought to be important for their secretion were also found at the C terminus of FrpA. The secretion of FrpA from E. coli by heterologous RTX transport proteins further demonstrates the relation of the FrpA protein to RTX toxins.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry,metabolism Bacterial Proteins/analysis Carrier Proteins/analysis Cytotoxins/metabolism Escherichia coli/metabolism Escherichia coli Proteins Hemolysin Proteins/analysis Iron-Binding Proteins Membrane Proteins Membrane Transport Proteins Molecular Sequence Data Neisseria meningitidis/metabolism Periplasmic Binding Proteins
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Cytotoxins Escherichia coli Proteins Hemolysin Proteins HlyD protein, E coli Hlyb protein, Bacteria Iron-Binding Proteins Membrane Proteins Membrane Transport Proteins Periplasmic Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thompson S A
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
Sparling P F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.7.2906-2911.1993
Published
1993-07-00
Pages
2906-11
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC280938
Subset
IM
Grants
NIAID NIH HHS · AI07001 · United States
NIAID NIH HHS · AI26837 · United States
External Links
PubMed source DOI: 10.1128/iai.61.7.2906-2911.1993 PMC Full Text View journal details
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