Home LiteratureArticle Details
PMID: 8539288 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves.

Plant physiology ·Vol. 109 ·No. 4 ·1995-12-00 ·Pages 1199-205

Verwoerd TC, van Paridon PA, van Ooyen AJ, van Lent JW, Hoekema A, Pen J

Abstract

Phytase from Aspergillus niger increases the availability of phosphorus from feed for monogastric animals by releasing phosphate from the substrate phytic acid. A phytase cDNA was constitutively expressed in transgenic tobacco (Nicotiana tabacum) plants. Secretion of the protein to the extracellular fluid was established by use of the signal sequence from the tobacco pathogen-related protein S. The specific phytase activity in isolated extracellular fluid was found to be approximately 90-fold higher than in total leaf extract, showing that the enzyme was secreted. This was confirmed by use of immunolocalization. Despite differences in glycosylation, specific activities of tobacco and Aspergillus phytase were identical. Phytase was found to be biologically active and to accumulate in leaves up to 14.4% of total soluble protein during plant maturation. Comparison of phytase accumulation and relative mRNA levels showed that phytase stably accumulated in transgenic leaves during plant growth.

MeSH Terms
6-Phytase/analysis,biosynthesis,genetics Amino Acid Sequence Aspergillus niger/enzymology,genetics Blotting, Western Extracellular Space/enzymology Gene Expression Glycoside Hydrolases Microscopy, Immunoelectron Molecular Sequence Data Plant Leaves Plants, Genetically Modified Plants, Toxic Plasmids Protein Sorting Signals/chemistry Recombinant Proteins/analysis,biosynthesis,chemistry Tobacco
Chemicals
Protein Sorting Signals Recombinant Proteins 6-Phytase Glycoside Hydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Verwoerd T C
MOGEN International N.V., Leiden, The Netherlands.
van Paridon P A
van Ooyen A J
van Lent J W
Hoekema A
Pen J
References (20)
20 references, click to expand
  1. Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence.
    Nature. 1987 Feb 12-18;325(6105):622-5 PMID: 3492677
  2. Expression of a chemically synthesized gene for human epidermal growth factor under the control of cauliflower mosaic virus 35S promoter in transgenic tobacco.
    Biosci Biotechnol Biochem. 1993 Sep;57(9):1477-81 PMID: 7764219
  3. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.
    Science. 1987 Jun 5;236(4806):1299-302 PMID: 17770331
  4. Deglycosylation of glycoproteins by trifluoromethanesulfonic acid.
    Anal Biochem. 1981 Nov 15;118(1):131-7 PMID: 6175244
  5. The expression, localization, and effect of a human interferon in plants.
    Virology. 1989 Sep;172(1):213-22 PMID: 2773316
  6. Codon usage in higher plants, green algae, and cyanobacteria.
    Plant Physiol. 1990 Jan;92(1):1-11 PMID: 16667228
  7. Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants.
    Nucleic Acids Res. 1988 Oct 11;16(19):9267-83 PMID: 3174450
  8. Structural characterization of a rice actin gene.
    Plant Mol Biol. 1990 Feb;14(2):163-71 PMID: 2101689
  9. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  10. Stable Accumulation of Modified 2S Albumin Seed Storage Proteins with Higher Methionine Contents in Transgenic Plants.
    Plant Physiol. 1990 Nov;94(3):970-9 PMID: 16667878
  11. Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
    Nucleic Acids Res. 1984 Sep 25;12(18):7057-70 PMID: 6207484
  12. Post-transcriptional regulation of nuclear-encoded genes in higher plants: the roles of mRNA stability and translation.
    Plant Mol Biol. 1993 Dec;23(6):1091-104 PMID: 8292775
  13. Production of antibodies in transgenic plants.
    Nature. 1989 Nov 2;342(6245):76-8 PMID: 2509938
  14. Complete nucleotide sequence of alfalfa mosaic virus RNA 4.
    Nucleic Acids Res. 1980 May 24;8(10):2213-23 PMID: 7433090
  15. A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin.
    Nature. 1986 May 29-Jun 4;321(6069):531-2 PMID: 3713832
  16. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  17. Localization of the 34 kDa polyhedron envelope protein in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus.
    Arch Virol. 1990;111(1-2):103-14 PMID: 2109589
  18. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  19. A small-scale procedure for the rapid isolation of plant RNAs.
    Nucleic Acids Res. 1989 Mar 25;17(6):2362 PMID: 2468132
  20. Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction.
    Biotechnology (N Y). 1992 Mar;10(3):292-6 PMID: 1368101
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.109.4.1199
Published
1995-12-00
Pages
1199-205
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC157650
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.109.4.1199 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2