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PMID: 8540694 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sterol compositions and susceptibilities to amphotericin B of environmental Cryptococcus neoformans isolates are changed by murine passage.

Antimicrobial agents and chemotherapy ·Vol. 39 ·No. 9 ·1995-09-00 ·Pages 1934-7

Currie B, Sanati H, Ibrahim AS, Edwards JE, Casadevall A, Ghannoum MA

Abstract

Previous studies have shown that sequential isolates from patients with persistent Cryptococcus neoformans meningoencephalitis can vary in sterol composition and susceptibility to antifungal drugs. To investigate the potential of host factors as mediators of this phenomenon, we compared fungal susceptibilities of environmental and clinical isolates from a limited geographic area. Clinical isolates were less susceptible to amphotericin B than environmental isolates. Five environmental isolates were passaged through BALB/c murine hosts; the passaged isolates had changes in sterol composition and reduced amphotericin B susceptibilities relative to those of the parent isolates. In contrast, murine passage of these isolates did not alter their susceptibilities to fluconazole. The results confirm that changes in sterol composition and antifungal susceptibility can occur in vivo as a result of host factors and suggest that human infection can result in selection of variants with reduced susceptibilities to amphotericin B.

MeSH Terms
Amphotericin B/pharmacology Animals Antifungal Agents/pharmacology Chromatography, Gas Columbidae Cryptococcus neoformans/drug effects,metabolism Feces/microbiology Fluconazole/pharmacology Mice Mice, Inbred BALB C Microbial Sensitivity Tests Sterols/metabolism
Chemicals
Antifungal Agents Sterols Amphotericin B Fluconazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Currie B
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Sanati H
Ibrahim A S
Edwards J E
Casadevall A
Ghannoum M A
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.39.9.1934
Published
1995-09-00
Pages
1934-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC162859
Subset
IM
Grants
NIAID NIH HHS · AI 31696-01 · United States
NIAID NIH HHS · R01-AI13342 · United States
NIAID NIH HHS · R01-AI33774 · United States
External Links
PubMed source DOI: 10.1128/AAC.39.9.1934 PMC Full Text View journal details
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