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PMID: 8540734 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequences of conserved region in the A subunit of DNA gyrase from nine species of the genus Mycobacterium: phylogenetic analysis and implication for intrinsic susceptibility to quinolones.

Antimicrobial agents and chemotherapy ·Vol. 39 ·No. 9 ·1995-09-00 ·Pages 2145-9

Guillemin I, Cambau E, Jarlier V

Abstract

The sequences of a conserved region in the A subunit of DNA gyrase corresponding to the quinolone resistance-determining region were determined for nine mycobacterial species and were compared. Although the nucleotide sequences were highly conserved, they clearly differentiated one species from another. The results of the phylogenetic analysis based on the sequences of the quinolone resistance-determining regions were compared with those provided by the 16S rRNA sequences. Deduced amino acid sequences were identical within the nine species except for amino acid 83, which was frequently involved in acquired resistance to quinolones in many genera, including mycobacteria. The presence at position 83 of an alanine for seven mycobacterial species (M. tuberculosis, M. bovis BCG, M. leprae, M. avium, M. kansasii, M. chelonae, and M. smegmatis) and of a serine for the two remaining mycobacterial species (M. fortuitum and M. aurum) correlated well with the MICs of ofloxacin for both groups of species, suggesting the role of this residue in intrinsic susceptibility to quinolones in mycobacteria.

MeSH Terms
4-Quinolones Amino Acid Sequence Anti-Infective Agents/pharmacology Base Sequence Conserved Sequence DNA Topoisomerases, Type II/biosynthesis,genetics DNA, Bacterial/metabolism Molecular Sequence Data Mycobacterium/enzymology,genetics,growth & development Phylogeny Polymerase Chain Reaction
Chemicals
4-Quinolones Anti-Infective Agents DNA, Bacterial DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guillemin I
Laboratoire de Recherche Moléculaire sur les Antibiotiques, Université Paris VI, Faculté de Médecine Pitié-Salpêtrière, France.
Cambau E
Jarlier V
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.39.9.2145
Published
1995-09-00
Pages
2145-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC162899
Subset
IM
Databases
GENBANK
X87117, X87118, X87119, X87120, X87121, X87122, X87123, X87124, X87125
External Links
PubMed source DOI: 10.1128/AAC.39.9.2145 PMC Full Text View journal details
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