Home LiteratureArticle Details
PMID: 8544833 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of spontaneous mutations at the scarlet locus of Drosophila melanogaster.

Molecular & general genetics : MGG ·Vol. 249 ·No. 6 ·1995-12-20 ·Pages 673-81

ten Have JF, Green MM, Howells AJ

Abstract

Six spontaneous mutations of the scarlet (st) locus of Drosophila melanogaster have been studied at the molecular level. Two of the mutants (st1 and stsp) arose in laboratory populations, while the other four (stcob, stct89, stdct and stdv) were isolated from natural populations. In five of these there is a DNA insertion within the st region and in four cases the insertion has been identified as being a transposable element; these include the retrotransposons 412 and B104/roo, and also jockey a member of the LINE family. In the other case (stdct), the insertion appears to consist of partially duplicated st sequences. In two of the mutants (st1 and stdv) the same transposable element (412) has inserted in the same orientation at exactly the same site within the st gene. The transposable element insertions are found in intron and exon regions of the st gene and also in the putative upstream regulatory region; insertions located in introns or exons result in the production of truncated st transcripts. The results show that the same types of transposable elements that cause spontaneous mutation in laboratory stocks of D. melanogaster also cause mutation in the wild.

MeSH Terms
ATP-Binding Cassette Transporters/genetics Animals Animals, Laboratory Animals, Wild Base Sequence DNA Transposable Elements/genetics Drosophila Proteins Drosophila melanogaster/genetics Exons/genetics Genes, Insect Introns/genetics Molecular Probe Techniques Molecular Sequence Data Mutagenesis Mutagenesis, Insertional Nucleic Acid Hybridization Polymerase Chain Reaction RNA, Messenger/genetics Sequence Analysis, DNA Sequence Deletion
Chemicals
ATP-Binding Cassette Transporters DNA Transposable Elements Drosophila Proteins RNA, Messenger st protein, Drosophila
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
ten Have J F
Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT, Australia.
Green M M
Howells A J
References (36)
36 references, click to expand
  1. Genetic instability in Drosophila melanogaster: the induction of specific chromosome 2 deletions by MR elements.
    Genetics. 1979 Jul;92(3):823-32 PMID: 119664
  2. Characterization of MR (P) strains of Drosophila melanogaster: the number of intact P elements and their genetic effect.
    Genet Res. 1991 Dec;58(3):211-23 PMID: 1666390
  3. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.
    Nucleic Acids Res. 1988 Aug 25;16(16):8186 PMID: 3047679
  4. Restriction-map variation in natural populations of Drosophila melanogaster: white-locus region.
    Mol Biol Evol. 1987 Nov;4(6):651-63 PMID: 2895415
  5. Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster.
    Cell. 1982 Sep;30(2):529-41 PMID: 6291773
  6. On the identification of the rosy locus DNA in Drosophila melanogaster: intragenic recombination mapping of mutations associated with insertions and deletions.
    Genetics. 1986 Apr;112(4):755-67 PMID: 3007280
  7. The molecular analysis of brown eye color mutations isolated from geographically discrete populations of Drosophila melanogaster.
    Mol Gen Genet. 1995 Apr 20;247(2):164-8 PMID: 7753025
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Partial revertants of the transposable element-associated suppressible allele white-apricot in Drosophila melanogaster: structures and responsiveness to genetic modifiers.
    Genetics. 1988 Feb;118(2):221-34 PMID: 2834265
  10. Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster.
    Genetics. 1986 Dec;114(4):1165-90 PMID: 3026893
  11. Molecular mapping of the rosy locus in Drosophila melanogaster.
    Genetics. 1986 Apr;112(4):769-83 PMID: 2420682
  12. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.
    J Mol Biol. 1983 Jul 25;168(1):17-33 PMID: 6410077
  13. Hybrid dysgenesis-induced revertants of insertions at the 5' end of the rudimentary gene in Drosophila melanogaster: transposon-induced control mutations.
    Genetics. 1986 Sep;114(1):165-82 PMID: 3021567
  14. Molecular cloning of lethal(2)giant larvae, a recessive oncogene of Drosophila melanogaster.
    EMBO J. 1985 Jun;4(6):1551-7 PMID: 3928370
  15. Nucleotide sequence of terminal repeats of 412 transposable elements of Drosophila melanogaster. A similarity to proviral long terminal repeats and its implications for the mechanism of transposition.
    J Mol Biol. 1981 Dec 25;153(4):897-915 PMID: 6283088
  16. Cloning and characterization of the scarlet gene of Drosophila melanogaster.
    Genetics. 1989 Jul;122(3):595-606 PMID: 2503416
  17. Purification of messenger RNA and heterogeneous nuclear RNA containing poly(a) sequences.
    Methods Enzymol. 1974;29:431-43 PMID: 4369093
  18. Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6455-9 PMID: 2842772
  19. Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3938-42 PMID: 2836866
  20. The Drosophila mobile element jockey belongs to LINEs and contains coding sequences homologous to some retroviral proteins.
    Gene. 1988 Oct 30;70(2):253-62 PMID: 2463954
  21. Effects of transposable element insertions on RNA encoded by the white gene of Drosophila.
    Cell. 1984 Sep;38(2):471-81 PMID: 6088083
  22. Genetic and functional analysis of tryptophan transport in Malpighian tubules of Drosophila.
    Biochem Genet. 1980 Dec;18(11-12):1109-30 PMID: 6788034
  23. Transposable elements in Drosophila melanogaster.
    Oxf Surv Eukaryot Genes. 1986;3:1-62 PMID: 2855303
  24. Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3490-3 PMID: 16592430
  25. Evidence that white-blood is a novel type of temperature-sensitive mutation resulting from temperature-dependent effects of a transposon insertion on formation of white transcripts.
    EMBO J. 1986 Dec 1;5(12):3343-51 PMID: 3028781
  26. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
    Nucleic Acids Res. 1991 Mar 11;19(5):1154 PMID: 2020552
  27. The nature of unstable insertion mutations and reversions in the locus cut of Drosophila melanogaster: molecular mechanism of transposition memory.
    EMBO J. 1985 Dec 30;4(13B):3781-7 PMID: 16453656
  28. Genetic applications of an inverse polymerase chain reaction.
    Genetics. 1988 Nov;120(3):621-3 PMID: 2852134
  29. A detailed developmental and structural study of the transcriptional effects of insertion of the Copia transposon into the white locus of Drosophila melanogaster.
    Genetics. 1985 Nov;111(3):495-515 PMID: 2414153
  30. Variation at the 87A heat shock locus in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5350-4 PMID: 16593362
  31. Defective P element insertions affect the expression of sn-glycerol-3-phosphate dehydrogenase alleles in natural populations of Drosophila melanogaster.
    Proc Biol Sci. 1993 Jan 22;251(1330):39-45 PMID: 8094564
  32. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  33. Structure and transcription of the Drosophila melanogaster vermilion gene and several mutant alleles.
    Mol Cell Biol. 1990 Apr;10(4):1423-31 PMID: 2108317
  34. Genomic sequences with homology to the P element of Drosophila melanogaster occur in the blowfly Lucilia cuprina.
    Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10753-7 PMID: 1332056
  35. Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678-82 PMID: 6300868
  36. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00418037
Published
1995-12-20
Pages
673-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00418037
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2