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PMID: 8551582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses.

Journal of virology ·Vol. 70 ·No. 2 ·1996-02-00 ·Pages 1213-7

Turelli P, Pétursson G, Guiguen F, Mornex JF, Vigne R, Quérat G

Abstract

The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measure the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages.

MeSH Terms
Animals Arthritis-Encephalitis Virus, Caprine/enzymology,physiology Base Sequence Cells, Cultured DNA, Viral Humans Macrophages/cytology Molecular Sequence Data Mutation Pyrophosphatases/genetics,metabolism Sequence Homology, Amino Acid Thymine Nucleotides/metabolism Virus Replication/physiology Visna-maedi virus/enzymology,physiology
Chemicals
DNA, Viral Thymine Nucleotides Pyrophosphatases dUTP pyrophosphatase thymidine 5'-triphosphate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Turelli P
INSERM U372, Marseille, France.
Pétursson G
Guiguen F
Mornex J F
Vigne R
Quérat G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.2.1213-1217.1996
Published
1996-02-00
Pages
1213-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189930
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.2.1213-1217.1996 PMC Full Text View journal details
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