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PMID: 8557045 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes.

The EMBO journal ·Vol. 14 ·No. 24 ·1995-12-15 ·Pages 6258-67

Scott KS, Geyer PK

Abstract

The suppressor of Hairy-wing [su(Hw)] protein mediates the mutagenic effects of the gypsy retrotransposon by blocking enhancer activity. These repressive effects are general, can occur over long distances and require that the su(Hw) protein is bound between the affected enhancer and promoter. The effects of the su(Hw) binding region on yolk protein (yp) gene expression were determined. These genes are regulated by shared enhancers in the intergenic region, which provided a method to examine whether an enhancer blocked by the su(Hw) protein remained functional. We demonstrate that a blocked enhancer is completely active, supporting the proposal that the su(Hw) protein is an insulator protein that acts by forming a new boundary in a pre-existing chromatin domain, thereby preventing the interaction of regulatory elements located upstream of the insertion site with the promoter. In addition, we found that yp promoter function is not diminished by sharing enhancers, suggesting that these enhancers are not rate limiting for transcriptional activation. Lastly, our data indicate that yp promoter activity is interdependent, such that transcription from one promoter influences the level of activity of the linked promoter.

MeSH Terms
Alcohol Dehydrogenase/genetics,metabolism Animals Base Sequence Cloning, Molecular DNA Primers/genetics DNA-Binding Proteins/genetics,physiology Drosophila/genetics,metabolism Drosophila Proteins Egg Proteins/genetics Enhancer Elements, Genetic Female Genes, Insect Male Models, Biological Molecular Sequence Data Nuclear Proteins/genetics,physiology Promoter Regions, Genetic Repressor Proteins Retroelements Tissue Distribution Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
DNA Primers DNA-Binding Proteins Drosophila Proteins Egg Proteins Nuclear Proteins Repressor Proteins Retroelements su(Hw) protein, Drosophila Alcohol Dehydrogenase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Scott K S
Department of Biochemistry, University of Iowa, College of Medicine, Iowa City 52242, USA.
Geyer P K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-12-15
Pages
6258-67
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394750
Subset
IM
Grants
NIGMS NIH HHS · GM42539 · United States
External Links
PubMed source PMC Full Text View journal details
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