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PMID: 85633 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Thermonuclease seroinhibition test for distinguishing Staphylococcus aureus from other coagulase-positive staphylococci.

Journal of clinical microbiology ·Vol. 9 ·No. 1 ·1979-01-00 ·Pages 141-3

Victor R, Lachica F, Jang SS, Hoeprich PD

Abstract

Since coagulase-positive staphylococci from animals are heterogeneous, another test is necessary to distinguish Staphylococcus aureus from them. Staphylococcal thermonucleases appear to be heterogeneous; antisera raised against S. aureus isolated from humans inhibit thermonuclease activity as demonstrated by the metachromatic well-agar diffusion method. The serological specificity of the thermonuclease elaborated by S. aureus of human origin was demonstrated using three antisera and 407 strains of staphylococci from diverse human and animal sources.

MeSH Terms

Animals Coagulase/biosynthesis Epitopes Hot Temperature Humans Immunodiffusion/methods Micrococcal Nuclease/biosynthesis,immunology Staphylococcus/enzymology Staphylococcus aureus/classification,enzymology

Chemicals

Coagulase Epitopes Micrococcal Nuclease

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Victor R

Lachica F

Jang S S

Hoeprich P D

References (12)

12 references, click to expand

Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci.
Arch Microbiol. 1976 Nov 2;110(23):263-70 PMID: 1015951
Coagulase and heat-resistant nuclease producing Staphylococcus epidermidis strains from animals.
J Appl Bacteriol. 1975 Oct;39(2):197-207 PMID: 1238391
Characteristics of Staphylococcus aureus strains isolated from different animal species.
Res Vet Sci. 1976 Nov;21(3):284-91 PMID: 140452
Correlation between the antigenic and catalytic properties of staphylococcal nuclease.
J Biol Chem. 1969 Feb 10;244(3):943-50 PMID: 4181374
Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests.
Appl Microbiol. 1973 Mar;25(3):496-7 PMID: 4572898
Convenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique.
Appl Microbiol. 1972 Dec;24(6):920-3 PMID: 4631104
Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.
Appl Microbiol. 1971 Apr;21(4):585-7 PMID: 4930272
[The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification].
Zentralbl Bakteriol Orig A. 1971 Jun;217(2):176-82 PMID: 5560788
Nuclease production and lysostaphin susceptibility of Staphylococcus aureus and other catalase-positive cocci.
Appl Microbiol. 1971 May;21(5):823-6 PMID: 5574317
Differentiation of strains of Staphylococcus epidermidis isolated from bovine udders.
J Gen Microbiol. 1967 May;47(2):273-87 PMID: 6045665
Deoxyribonucleic acid base composition and biochemical properties of certain coagulase-negative enterotoxigenic cocci.
Appl Microbiol. 1975 Feb;29(2):152-8 PMID: 803812
Some properties of coagulase-negative deoxyribonuclease-producing strains of staphylococci from human infections.
Med Microbiol Immunol. 1976 Jun 1;162(2):143-52 PMID: 934023

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.9.1.141-143.1979

Published

1979-01-00

Pages

141-3

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC272971

Subset

External Links

PubMed source DOI: 10.1128/jcm.9.1.141-143.1979 PMC Full Text View journal details

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