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PMID: 8577762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 3 ·1996-02-06 ·Pages 1320-4

Miyake S, Makimura M, Kanegae Y, Harada S, Sato Y, Takamori K, Tokuda C, Saito I

Abstract

An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the full-length Ad genome cloned in a cassette cosmid. The cassette bearing the expression unit was then cotransfected into human embryonic kidney 293 cells together with the Ad DNA-terminal protein complex digested at several sites with Eco T22I or Ase I/EcoRI. The use of the parent Ad DNA-terminal protein complex instead of the deproteinized Ad genome DNA allowed very efficient recovery of the desired recombinant Ad, and the above restriction digestion drastically reduced regeneration of the parent virus. Several hundred virus clones were readily obtained in each experiment, and about 70% of the clones were the desired recombinant viruses. Furthermore, because the cassette contained the full-length Ad genome, any position of the genome could be easily modified to develop a new vector design. We established construction systems for two types of Ad vectors, the E1-substitution type and the E4-insertion type. This method may greatly facilitate the application of recombinant Ads and should be useful for further improvement of Ad vectors.

MeSH Terms
Adenovirus E4 Proteins/biosynthesis Adenoviruses, Human/genetics,metabolism Base Sequence Cell Line Cosmids DNA, Viral/metabolism Embryo, Mammalian Gene Expression Genetic Vectors Genome, Viral Humans Kidney Molecular Sequence Data Mutagenesis, Insertional Oligodeoxyribonucleotides Recombination, Genetic Restriction Mapping Viral Proteins/metabolism
Chemicals
Adenovirus E4 Proteins DNA, Viral Oligodeoxyribonucleotides Viral Proteins terminal protein, adenovirus
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Miyake S
Laboratory of Molecular Genetics, University of Tokyo, Japan.
Makimura M
Kanegae Y
Harada S
Sato Y
Takamori K
Tokuda C
Saito I
References (39)
39 references, click to expand
  1. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.
    Proc Natl Acad Sci U S A. 1990 Dec;87(24):9524-8 PMID: 2175903
  2. Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system.
    Gene. 1990 Jul 16;91(2):217-23 PMID: 2210382
  3. Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line.
    Mol Cell Biol. 1991 Nov;11(11):5527-33 PMID: 1656233
  4. Efficient selection for high-expression transfectants with a novel eukaryotic vector.
    Gene. 1991 Dec 15;108(2):193-9 PMID: 1660837
  5. The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen.
    J Immunol. 1992 Feb 15;148(4):1274-9 PMID: 1371136
  6. Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells.
    J Virol. 1992 Mar;66(3):1425-31 PMID: 1310756
  7. A possible regulatory role for conserved promoter motifs in an adult-specific muscle myosin gene from mouse.
    J Biol Chem. 1992 Aug 25;267(24):16957-67 PMID: 1324916
  8. Efficient adenovirus-mediated transfer of a human minidystrophin gene to skeletal muscle of mdx mice.
    Nature. 1993 Feb 18;361(6413):647-50 PMID: 8437625
  9. An adenovirus vector for gene transfer into neurons and glia in the brain.
    Science. 1993 Feb 12;259(5097):988-90 PMID: 8382374
  10. Adenovirus-mediated gene transfer transiently corrects the chloride transport defect in nasal epithelia of patients with cystic fibrosis.
    Cell. 1993 Oct 22;75(2):207-16 PMID: 7691415
  11. Efficient manipulation of the human adenovirus genome as an infectious yeast artificial chromosome clone.
    Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6186-90 PMID: 8016135
  12. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3.
    Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8802-6 PMID: 8090727
  13. Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis.
    Nat Genet. 1994 Sep;8(1):42-51 PMID: 7527271
  14. Adenovirus-mediated transfer of the HST-1 (FGF4) gene induces increased levels of platelet count in vivo.
    Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12368-72 PMID: 7809043
  15. Characterization of measles viruses isolated after measles vaccination.
    Vaccine. 1995 Mar;13(4):370-2 PMID: 7793133
  16. Gene targeting with a replication-defective adenovirus vector.
    J Virol. 1995 Oct;69(10):6180-90 PMID: 7666520
  17. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
    Nucleic Acids Res. 1995 Oct 11;23(19):3816-21 PMID: 7479022
  18. The infectivity of adenovirus 5 DNA-protein complex.
    Virology. 1976 Dec;75(2):442-56 PMID: 1032904
  19. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  20. Isolation of deletion and substitution mutants of adenovirus type 5.
    Cell. 1978 Jan;13(1):181-8 PMID: 620421
  21. Physical mapping of a large-plaque mutation of adenovirus type 2.
    J Virol. 1979 Nov;32(2):623-8 PMID: 501803
  22. The infectivity of adenovirus genomes lacking DNA sequences from their left-hand termini.
    Nucleic Acids Res. 1982 Sep 11;10(17):5105-19 PMID: 6292829
  23. Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome.
    J Virol. 1985 Jun;54(3):711-9 PMID: 3999192
  24. Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.
    EMBO J. 1985 Oct;4(10):2561-8 PMID: 3876930
  25. Bacteriophage P1 cre gene and its regulatory region. Evidence for multiple promoters and for regulation by DNA methylation.
    J Mol Biol. 1986 Jan 20;187(2):197-212 PMID: 3486297
  26. Charomids: cosmid vectors for efficient cloning and mapping of large or small restriction fragments.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8664-8 PMID: 3022301
  27. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin.
    Nature. 1986 Nov 6-12;324(6092):73-6 PMID: 3491322
  28. Molecular cloning and expression of an IL-6 signal transducer, gp130.
    Cell. 1990 Dec 21;63(6):1149-57 PMID: 2261637
  29. SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.
    Mol Cell Biol. 1988 Jan;8(1):466-72 PMID: 2827008
  30. Structure of the human ornithine transcarbamylase gene.
    J Biochem. 1988 Feb;103(2):302-8 PMID: 2836378
  31. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor.
    Science. 1988 Aug 12;241(4867):825-8 PMID: 3136546
  32. Cloning and expression of human arylsulfatase A.
    J Biol Chem. 1989 Jan 15;264(2):1252-9 PMID: 2562955
  33. A protein kinase C cDNA without the regulatory domain is active after transfection in vivo in the absence of phorbol ester.
    Mol Cell Biol. 1989 Feb;9(2):831-6 PMID: 2785241
  34. Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA.
    J Cell Biol. 1989 Sep;109(3):1173-84 PMID: 2504728
  35. A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis.
    Mol Cell Biol. 1990 May;10(5):2261-8 PMID: 2183027
  36. High level expression of the envelope glycoproteins of the human immunodeficiency virus type I in presence of rev gene using helper-independent adenovirus type 7 recombinants.
    Virology. 1990 Apr;175(2):535-47 PMID: 2183470
  37. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src.
    Science. 1990 May 11;248(4956):727-30 PMID: 1692159
  38. Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. III. Nucleotide sequence of the hemagglutinin gene.
    Virus Genes. 1990 Jul;4(2):163-72 PMID: 2402882
  39. A murine model for type III tyrosinemia: lack of immunologically detectable 4-hydroxyphenylpyruvic acid dioxygenase enzyme protein in a novel mouse strain with hypertyrosinemia.
    Am J Hum Genet. 1991 Apr;48(4):704-9 PMID: 2014797
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.3.1320
Published
1996-02-06
Pages
1320-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40078
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.3.1320 PMC Full Text View journal details
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