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PMID: 8605883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.

The EMBO journal ·Vol. 15 ·No. 5 ·1996-03-01 ·Pages 1132-41

Hayes R, Kudla J, Schuster G, Gabay L, Maliga P, Gruissem W

Abstract

In the absence of efficient transcription termination correct 3'-end processing is an essential step in the synthesis of stable chloroplast mRNAs in higher plants. We show here that 3'-end processing in vitro involves endonucleolytic cleavage downstream from the mature terminus, followed by exonucleolytic processing to a stem-loop within the 3'-untranslated region. These processing steps require a high molecular weight complex that contains both endoribonucleases and an exoribonuclease. In the presence of ancillary RNA binding proteins the complex correctly processes the 3'-end of precursor RNA. In the absence of these ancillary proteins 3'-end maturation is prevented and plastid mRNAs are degraded. Based on these results we propose a novel mechanism for the regulation of mRNA 3'-end processing and stability in chloroplasts.

MeSH Terms
Amino Acid Sequence Chloroplasts/metabolism Cytochrome b Group/genetics Cytochrome b6f Complex Endoribonucleases/metabolism Molecular Sequence Data Molecular Weight Plant Proteins/metabolism Plastids/metabolism Polyribonucleotide Nucleotidyltransferase/genetics,metabolism RNA Processing, Post-Transcriptional RNA, Messenger/metabolism RNA, Plant/metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid Spinacia oleracea/genetics,metabolism
Chemicals
Cytochrome b Group Plant Proteins RNA, Messenger RNA, Plant RNA-Binding Proteins Ribonucleoproteins petD protein, cytochrome b(6)f complex Cytochrome b6f Complex Polyribonucleotide Nucleotidyltransferase Endoribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hayes R
Waksman Institute, Rutgers University, Piscataway, NJ 08855-0759, USA.
Kudla J
Schuster G
Gabay L
Maliga P
Gruissem W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-03-01
Pages
1132-41
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450011
Subset
IM
Databases
GENBANK
U52048
External Links
PubMed source PMC Full Text View journal details
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