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PMID: 8610150 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 7 ·1996-04-02 ·Pages 2958-63

Kovalenko OV, Plug AW, Haaf T, Gonda DK, Ashley T, Ward DC, Radding CM, Golub EI

Abstract

Hsubc9, a human gene encoding a ubiquitin-conjugating enzyme, has been cloned. The 18-kDa HsUbc9 protein is homologous to the ubiquitin-conjugating enzymes Hus5 of Schizosaccharomyces pombe and Ubc9 of Saccharomyces cerevisiae. The Hsubc9 gene complements a ubc9 mutation of S. cerevisiae. It has been mapped to chromosome 16p13.3 and is expressed in many human tissues, with the highest levels in testis and thymus. According to the Ga14 two-hybrid system analysis, HsUbc9 protein interacts with human recombination protein Rad51. A mouse homolog, Mmubc9, encodes an amino acid sequence that is identical to the human protein. In mouse spermatocytes, MmUbc9 protein, like Rad51 protein, localizes in synaptonemal complexes, which suggests that Ubc9 protein plays a regulatory role in meiosis.

MeSH Terms
Amino Acid Sequence Animals Blotting, Western Chromosome Mapping Chromosomes, Human DNA-Binding Proteins/analysis,biosynthesis,metabolism Female Humans Immunohistochemistry In Situ Hybridization, Fluorescence Ligases/analysis,biosynthesis,metabolism Male Mice Molecular Sequence Data Organ Specificity Rad51 Recombinase Recombinant Proteins/analysis,biosynthesis,metabolism Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Spermatocytes/metabolism Synaptonemal Complex/physiology Ubiquitin-Conjugating Enzymes X Chromosome Y Chromosome
Chemicals
DNA-Binding Proteins RHP51 protein, S pombe Recombinant Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Ubiquitin-Conjugating Enzymes RAD51 protein, S cerevisiae RAD51 protein, human Rad51 Recombinase Rad51 protein, mouse Ligases ubiquitin-conjugating enzyme UBC9
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kovalenko O V
Department of Genetics, Yale University School of Medicine, New Haven CT 06510, USA.
Plug A W
Haaf T
Gonda D K
Ashley T
Ward D C
Radding C M
Golub E I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.7.2958
Published
1996-04-02
Pages
2958-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39742
Subset
IM
Grants
PHS HHS · 5R01H900272 · United States
NCI NIH HHS · CA39238 · United States
NIGMS NIH HHS · GM49779 · United States
Databases
GENBANK
U31933, U31934
External Links
PubMed source DOI: 10.1073/pnas.93.7.2958 PMC Full Text View journal details
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