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PMID: 8628215 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links.

Molecular & general genetics : MGG ·Vol. 250 ·No. 2 ·1996-02-05 ·Pages 162-8

Wolter R, Siede W, Brendel M

Abstract

The interstrand cross-link repair gene SNM1 of Saccharomyces cerevisiae was examined for regulation in response to DNA-damaging agents. Induction of SNM1-lacZ fusions was detected in response to nitrogen mustard, cis-platinum (II) diamine dichloride, UV light, and 8-methoxypsoralen + UVA, but not after heat-shock treatment or incubation with 2-dimethylaminoethylchloride, methylmethane sulfonate or 4-nitroquinoline-N-oxide. The promoter of SNM1 contains a 15 bp motif, which shows homology to the DRE2 box of the RAD2 promoter. Similar motifs have been found in promoter regions of other damage-inducible DNA repair genes. Deletion of this motif results in loss of inducibility of SNM1. Also, a putative negative upstream regulation sequence was found to be responsible for repression of constitutive transcription of SNM1. Surprisingly, no inducibility of SNM1 was found after treatment with DNA-damaging agents in strains without an intact DUN1 gene, while regulation seems unchanged in sad1 mutants.

MeSH Terms
Antineoplastic Agents/pharmacology Base Sequence Cisplatin/pharmacology Consensus Sequence/genetics Cross-Linking Reagents/chemistry,pharmacology DNA Damage DNA Repair/genetics DNA, Fungal/drug effects,radiation effects DNA-Binding Proteins/genetics Endodeoxyribonucleases Fungal Proteins/genetics Gene Expression Regulation, Fungal Methoxsalen/pharmacology Molecular Sequence Data Mutagenesis/genetics Nuclear Proteins Promoter Regions, Genetic/genetics Recombinant Fusion Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Deletion Sequence Homology, Nucleic Acid TATA Box/genetics Ultraviolet Rays beta-Galactosidase/genetics,metabolism
Chemicals
Antineoplastic Agents Cross-Linking Reagents DNA, Fungal DNA-Binding Proteins Fungal Proteins Nuclear Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Endodeoxyribonucleases PSO2 protein, S cerevisiae beta-Galactosidase Cisplatin Methoxsalen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wolter R
Institut für Mikrobiologie, Abt. Biologie für Mediziner, J.W. Goethe-Universität, Frankfurt am Main, FRG.
Siede W
Brendel M
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02174175
Published
1996-02-05
Pages
162-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02174175
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