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PMID: 8628321 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.

Molecular and cellular biology ·Vol. 16 ·No. 5 ·1996-05-00 ·Pages 2527-36

Waterham HR, de Vries Y, Russel KA, Xie W, Veenhuis M, Cregg JM

Abstract

We report the cloning of PER6, a gene essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. The PER6 sequence predicts that its product Per6p is a 52-kDa polypeptide with the cysteine-rich C3HC4 motif. Per6p has significant overall sequence similarity with the human peroxisome assembly factor PAF-1, a protein that is defective in certain patients suffering from the peroxisomal disorder Zellweger syndrome, and with car1, a protein required for peroxisome biogenesis and caryogamy in the filamentous fungus Podospora anserina. In addition, the C3HC4 motif and two of the three membrane-spanning segments predicted for Per6p align with the C3HC4 motifs and the two membrane-spanning segments predicted for PAF-1 and car1. Like PAF-1, Per6p is a peroxisomal integral membrane protein. In methanol- or oleic acid-induced cells of per6 mutants, morphologically recognizable peroxisomes are absent. Instead, peroxisomal remnants are observed. In addition, peroxisomal matrix proteins are synthesized but located in the cytosol. The similarities between Per6p and PAF-1 in amino acid sequence and biochemical properties, and between mutants defective in their respective genes, suggest that Per6p is the putative yeast homolog of PAF-1.

MeSH Terms
Amino Acid Sequence Antibodies Base Sequence Biomarkers Cytosol/metabolism Fungal Proteins/biosynthesis,chemistry,genetics Genes, Fungal Genotype Humans Membrane Proteins/biosynthesis,chemistry,genetics Microbodies/metabolism,physiology,ultrastructure Microscopy, Electron Mitochondria/metabolism Molecular Sequence Data Peroxisomal Biogenesis Factor 2 Pichia/genetics,physiology,ultrastructure Reading Frames Sequence Homology, Amino Acid Xylariales/genetics Zellweger Syndrome/genetics,metabolism
Chemicals
Antibodies Biomarkers Fungal Proteins Membrane Proteins PER6 protein, Pichia pastoris Peroxisomal Biogenesis Factor 2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Waterham H R
Department of Chemistry, Biochemistry, and Molecular Biology, Oregon Graduate Institute of Science and Technology, Portland, 97291-1000, USA.
de Vries Y
Russel K A
Xie W
Veenhuis M
Cregg J M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.5.2527
Published
1996-05-00
Pages
2527-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231242
Subset
IM
Grants
NIDDK NIH HHS · DK-43698 · United States
Databases
GENBANK
X96945
External Links
PubMed source DOI: 10.1128/MCB.16.5.2527 PMC Full Text View journal details
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