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PMID: 8636030 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Circular and linear plasmids of Lyme disease spirochetes have extensive homology: characterization of a repeated DNA element.

Journal of bacteriology ·Vol. 178 ·No. 8 ·1996-04-00 ·Pages 2287-98

Zückert WR, Meyer J

Abstract

We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A and ORF-B. Five additional ORFs can be found on the slightly less homologous flanking sequences: ORF-G on the opposite strand upstream of ORF-A, and ORF-C, ORF-D, ORF-E, and ORF-F downstream of ORF-B. The 4.6-kb-long element containing ORF-A through ORF-E is flanked by approximately 180-bp-long imperfect inverted repeats (IRs). The putative gene product of ORF-C displays homology to proteins involved in plasmid maintenance in a number of gram-positive and gram-negative bacteria. ORF-E features several short, highly homologous direct repeats. ORF-A, ORF-B, and ORF-D are homologous to three ORFs on a recently described 8.3-kb circular plasmid of Borrelia afzelii Ip21 that are flanked by similar IRs (J. J. Dunn, S. R. Buchstein, L.-L. Butler, S. Fisenne, D. S. Polin, B. N. Lade, and B. J. Luft, J. Bacteriol. 176:2706-2717,1994). ORF-C and ORF-E, however, are missing from this region on the Ip21 plasmid. Furthermore, the repeated DNA element as defined by the IRs is present in opposite orientations relative to the flanking sequences on the B31 and Ip21 plasmids.

MeSH Terms
Base Sequence Borrelia burgdorferi Group/classification,genetics Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames Plasmids/genetics Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zückert W R
Department of Preventive Dentistry and Oral Microbiology, University of Basel Dental Institute, Switzerland.
Meyer J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.8.2287-2298.1996
Published
1996-04-00
Pages
2287-98
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177937
Subset
IM
Databases
GENBANK
X87127, X87201, X87202
External Links
PubMed source DOI: 10.1128/jb.178.8.2287-2298.1996 PMC Full Text View journal details
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