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PMID: 8657104 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene.

Molecular and cellular biology ·Vol. 16 ·No. 4 ·1996-04-00 ·Pages 1316-25

Mink S, Kerber U, Klempnauer KH

Abstract

The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which activates the myelomonocyte-specific mim-1 gene, a natural myb target gene, by cooperating with members of the C/EBP transcription factor family. The finding that v-Myb, together with C/EBP, is sufficient to activate the mim-1 gene in heterologous cell types has implicated Myb and C/EBP as a bipartite molecular switch, which regulates the expression of myelomonocyte-specific genes. To understand the relationship between v-Myb and C/EBP in more detail, we have examined the molecular basis of the activation of the mim-1 promoter by v-Myb and C/EBPbeta, a member of the C/EBP transcription factor family highly expressed in myelomonocytic cells. We have identified a composite Myb and C/EBP response element which mediates synergistic activation of the mim-1 promoter by both factors and consists of closely spaced Myb- and C/EBP-binding sites. In vitro and in vivo protein-binding studies indicate that v-Myb and C/EBPbeta interact with each other via their DNA-binding domains. We show that this interaction is essential for the synergistic activation of the mim-1 promoter by v-Myb and C/EBPbeta. Our work therefore identifies C/EBPbeta as an interaction partner of v-Myb involved in myelomonocyte gene expression.

MeSH Terms
Acetyltransferases Animals Base Sequence Blotting, Western CCAAT-Enhancer-Binding Proteins Cell Line Chickens DNA-Binding Proteins/genetics,metabolism Genes, Reporter Molecular Sequence Data Nuclear Proteins/genetics,metabolism Oncogenes Precipitin Tests Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcriptional Activation
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Nuclear Proteins Proteins Transcription Factors Acetyltransferases mim-1 protein, Gallus gallus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mink S
Hans Spemann Laboratory, Max Planck Institute for Immunobiology, Freiburg, Germany.
Kerber U
Klempnauer K H
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46 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.4.1316
Published
1996-04-00
Pages
1316-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231115
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.4.1316 PMC Full Text View journal details
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