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PMID: 8698478 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of isp, a locus encoding an immunogenic secreted protein conserved among group A streptococci.

Infection and immunity ·Vol. 64 ·No. 7 ·1996-07-00 ·Pages 2548-55

McIver KS, Subbarao S, Kellner EM, Heath AS, Scott JR

Abstract

The protein Mga (mga), which is required for transcription of several virulence genes of group A streptococci (GAS), including the antiphagocytic M protein, was suggested to act as the response regulator element of a bacterial two-component pathway. To investigate whether a gene encoding a cognate sensor protein is located upstream of mga, 3.1 kb of DNA 5' of the mga translational start site was cloned from serotype M6 GAS strain JRS4. Sequence analysis of this region revealed two adjacent open reading frames, a previously described orf and a new locus, isp (immunogenic secreted protein), which could encode proteins of 9 and 59 kDa, respectively. Inactivation of either open reading frame had no significant effect on transcription of the gene encoding M protein (emm) under normal growth conditions, suggesting that neither isp nor orf is involved in the Mga regulatory circuit. A protein migrating at an apparent molecular weight of 65,000 was produced when isp was transcribed and translated in vitro. The predicted isp product (Isp) contains an amino-terminal signal sequence region homologous to that found in bacterial secreted proteins, and expression of isp in Escherichia coli resulted in the presence of Isp in the periplasmic fraction. Convalescent-phase serum from a patient with an active GAS infection recognized forms of Isp both from the periplasm of E. coli and the supernatant of a GAS strain. Both isp and orf are highly conserved among strains of GAS, as shown by hybridization analyses.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,immunology,metabolism Base Sequence Conserved Sequence DNA Primers/genetics DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Molecular Weight Sequence Homology, Amino Acid Streptococcus pyogenes/genetics,immunology Transcription Factors/genetics
Chemicals
Bacterial Proteins DNA Primers DNA, Bacterial Transcription Factors mry protein, Streptococcus pyogenes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McIver K S
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322, USA.
Subbarao S
Kellner E M
Heath A S
Scott J R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.7.2548-2555.1996
Published
1996-07-00
Pages
2548-55
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174109
Subset
IM
Grants
NIAID NIH HHS · R37-AI20723 · United States
Databases
GENBANK
U31811
External Links
PubMed source DOI: 10.1128/iai.64.7.2548-2555.1996 PMC Full Text View journal details
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