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PMID: 1977705 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli.

Infection and immunity ·Vol. 58 ·No. 11 ·1990-11-00 ·Pages 3594-600

Perez-Casal J, Swartley JS, Scott JR

Abstract

Some enterotoxigenic strains of Escherichia coli (ETEC) utilize the CS1 pilus for colonization of human intestinal epithelium. We have cloned the gene which encodes the major CS1 subunit and called it cooA (for coli surface antigen one). Hybridization showed that the ETEC strain from which it was cloned carried cooA on a plasmid different from the one encoding its positive regulator, rns. Based on the cooA DNA sequence, cleavage with signal peptidase would be expected to produce a mature protein of 15.2 kDa; a 16-kDa polypeptide that reacted with CS1-specific antiserum was observed on electrophoresis. At the protein level, there was 92% similarity and 55% identity between cooA and cfaB, the major colonization factor antigen I (CFA/I) antigen. However, CS1-specific antisera did not react with CfaB. No hybridization was seen between either of two different cooA probes and total DNA from ETEC strains expressing AFA-1, CFA/I, CS2, CS3, CS4, CS5, or CS6.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Bacterial Outer Membrane Proteins/genetics,immunology Bacterial Proteins/genetics,immunology Base Sequence Cloning, Molecular Cross Reactions DNA, Bacterial/chemistry Escherichia coli/genetics,immunology Fimbriae Proteins Fimbriae, Bacterial/metabolism Genes, Bacterial Humans Molecular Sequence Data Operon Sequence Homology, Nucleic Acid
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial colonization factor antigens Fimbriae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Perez-Casal J
Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322.
Swartley J S
Scott J R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.11.3594-3600.1990
Published
1990-11-00
Pages
3594-600
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313703
Subset
IM
Grants
NIAID NIH HHS · AI28470 · United States
Databases
GENBANK
M37148, M58550
External Links
PubMed source DOI: 10.1128/iai.58.11.3594-3600.1990 PMC Full Text View journal details
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