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PMID: 2687231 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a divergent M protein gene and an M protein-related gene family in Streptococcus pyogenes serotype 49.

Journal of bacteriology ·Vol. 171 ·No. 12 ·1989-12-00 ·Pages 6397-408

Haanes EJ, Cleary PP

Abstract

The antigenically variant M protein of Streptococcus pyogenes enhances virulence by promoting resistance to phagocytosis. The serum opacity factor (OF), produced by a subset of M serotypes, is also antigenically variant, and its antigenic variability exactly parallels that of M protein. OF-positive and OF-negative streptococci are also phenotypically distinguishable by a number of other criteria. In order to study the differences between OF-positive and OF-negative streptococci, we cloned and sequenced the type 49 M protein gene (emm49), the first to be cloned from an OF-positive strain. This gene showed evolutionary divergence from the OF-negative M protein genes studied previously. Furthermore, emm49 was part of a gene family, in contrast to the single-copy nature of previously characterized M protein genes.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Base Sequence Carrier Proteins Cloning, Molecular Gene Expression Genes, Bacterial Genetic Variation Information Systems Molecular Sequence Data Multigene Family Restriction Mapping Sequence Homology, Nucleic Acid Serotyping Software Streptococcus pyogenes/classification,genetics
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins streptococcal M protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haanes E J
Department of Microbiology, University of Minnesota, Minneapolis 55455.
Cleary P P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.12.6397-6408.1989
Published
1989-12-00
Pages
6397-408
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210527
Subset
IM
Grants
NIAID NIH HHS · AI16722 · United States
External Links
PubMed source DOI: 10.1128/jb.171.12.6397-6408.1989 PMC Full Text View journal details
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