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PMID: 8725223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Meiotic recombination initiated by a double-strand break in rad50 delta yeast cells otherwise unable to initiate meiotic recombination.

Genetics ·Vol. 143 ·No. 2 ·1996-06-00 ·Pages 741-54

Malkova A, Ross L, Dawson D, Hoekstra MF, Haber JE

Abstract

Meiotic recombination in Saccharomyces cerevisiae is initiated by double- strand breaks (DSBs). We have developed a system to compare the properties of meiotic DSBs with those created by the site-specific HO endonuclease. HO endonuclease was expressed under the control of the meiotic-specific SPO13 promoter, creating a DSB at a single site on one of yeast's 16 chromosomes. In Rad+ strains the times of appearance of the HO-induced DSBs and of subsequent recombinants are coincident with those induced by normal meiotic DSBs. Physical monitoring of DNA showed that SPO13: : HO induced gene conversions both in Rad+ and in rad50 delta cells that cannot initiate normal meiotic DSBs. We find that the RAD50 gene is important, but not essential, for recombination even after a DSB has been created in a meiotic cell. In rad50 delta cells, some DSBs are not repaired until a broken chromosome has been packaged into a spore and is subsequently germinated. This suggests that a broken chromosome does not signal an arrest of progression through meiosis. The recombination defect in rad50 delta diploids is not, however, meiotic specific, as mitotic rad50 diploids, experiencing an HO-induced DSB, exhibit similar departures from wild-type recombination.

MeSH Terms
DNA, Fungal/genetics Deoxyribonucleases, Type II Site-Specific/genetics Meiosis/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
DNA, Fungal Saccharomyces cerevisiae Proteins HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Malkova A
Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, USA.
Ross L
Dawson D
Hoekstra M F
Haber J E
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/143.2.741
Published
1996-06-00
Pages
741-54
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207333
Subset
IM
Grants
NIGMS NIH HHS · GM-29376 · United States
NIGMS NIH HHS · GM-40452 · United States
External Links
PubMed source DOI: 10.1093/genetics/143.2.741 PMC Full Text View journal details
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