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PMID: 8727898 Published · ppublish English Comparative Study Journal Article

Novel BOX repeat PCR assay for high-resolution typing of Streptococcus pneumoniae strains.

Journal of clinical microbiology ·Vol. 34 ·No. 5 ·1996-05-00 ·Pages 1176-9

van Belkum A, Sluijuter M, de Groot R, Verbrugh H, Hermans PW

Abstract

Typing data obtained by specifically targeting a single, high-stringency PCR at the pneumococcal BOX repeat element for 28 strains of Streptococcus pneumoniae completely corroborated the resolutions attained by five genotypic procedures as described by Hermans et al. (P.W.M. Hermans, M. Sluijter, T. Hoogenboezem, H. Heersma, A. van Belkum, and R. de Groot, J. Clin. Microbiol. 33:1606-1612, 1995). All pairs of strains, except one, derived from both the cerebrospinal fluid and blood of the same individual were shown to be identical. Moreover, other, epidemiologically unrelated isolates were demonstrated to be unique. Considering the combined data from the five typing techniques applied previously as the "gold standard," the single BOX PCR test demonstrated excellent resolving powers while maintaining epidemiological linkage.

MeSH Terms
Bacterial Typing Techniques Base Sequence DNA Fingerprinting DNA Primers/genetics DNA, Bacterial/genetics,isolation & purification Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction/methods Repetitive Sequences, Nucleic Acid Streptococcus pneumoniae/classification,genetics
Chemicals
DNA Primers DNA, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Belkum A
Department of Clinical Microbiology, University Hospital Rotterdam, Erasmus University, The Netherlands. [email protected]
Sluijuter M
de Groot R
Verbrugh H
Hermans P W
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.34.5.1176-1179.1996
Email
[email protected]
Published
1996-05-00
Pages
1176-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228977
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.34.5.1176-1179.1996 PMC Full Text View journal details
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