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PMID: 8756666 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 16 ·No. 9 ·1996-09-00 ·Pages 5081-90

Lei M, Kawasaki Y, Tye BK

Abstract

Mcm2, Mcm3, and Mcm5/Cdc46 are conserved proteins essential for the initiation of DNA synthesis at replication origins in Saccharomyces cerevisiae. The accumulation of these proteins in the nucleus before the onset of DNA synthesis suggests that they play a role in restricting DNA synthesis to once per cell cycle. In this work, we show that Mcm2, Mcm3, and Mcm5 self-interact and interact with one another to form complexes. Mcm2 and Mcm3 are abundant proteins, present in approximately 4 X 10(4) and 2 X 10(5) copies per cell, respectively. Reducing the dosage of Mcm2 by half results in diminished usage of specific replication origins. These results together suggest that a significant molar excess of Mcm proteins relative to replication origins is required for the proper initiation of all replication origins.

MeSH Terms
Cell Cycle Proteins/isolation & purification,metabolism Chromatography, Affinity Chromosomal Proteins, Non-Histone DNA Replication DNA, Fungal/biosynthesis,genetics Fungal Proteins/isolation & purification,metabolism Macromolecular Substances Recombinant Fusion Proteins/isolation & purification,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Cell Cycle Proteins Chromosomal Proteins, Non-Histone DNA, Fungal Fungal Proteins MCM5 protein, S cerevisiae Macromolecular Substances Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins MCM2 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lei M
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
Kawasaki Y
Tye B K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.9.5081
Published
1996-09-00
Pages
5081-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231509
Subset
IM
Grants
NIGMS NIH HHS · GM34190 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.9.5081 PMC Full Text View journal details
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