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PMID: 2233713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication.

Molecular and cellular biology ·Vol. 10 ·No. 11 ·1990-11-00 ·Pages 5707-20

Gibson SI, Surosky RT, Tye BK

Abstract

MCM3 is an essential gene involved in the maintenance of minichromosomes in yeast cells. It encodes a protein of 971 amino acids that shows striking homology to the Mcm2 protein. We have mapped the mcm3-1 mutation of the left arm of chromosome V approximately 3 kb centromere proximal of anp1. The mcm3-1 mutant was found to be thermosensitive for growth. Under permissive growth conditions, it was defective in minichromosome maintenance in an autonomously replicating sequence-specific manner and showed an increase in chromosome loss and recombination. Under nonpermissive conditions, mcm3-1 exhibited a cell cycle arrest phenotype, arresting at the large-bud stage with an undivided nucleus that had a DNA content of nearly 2n. These phenotypes are consistent with incomplete replication of the genome of the mcm3-1 mutant, possibly as a result of limited replication initiation at selective autonomously replicating sequences leading to cell cycle arrest before mitosis. The phenotype exhibited by the mcm3 mutant is very similar to that of mcm2, suggesting that the Mcm2 and Mcm3 protein may play interacting roles in DNA replication.

Related Genes
mcm3
MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Proteins Chromosomes, Fungal/physiology Cloning, Molecular DNA Replication Escherichia coli/genetics Fungal Proteins/genetics Genes, Fungal Genotype Kinetics Mitosis Molecular Sequence Data Mutation Oligonucleotide Probes Phenotype Plasmids Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/cytology,genetics,growth & development
Chemicals
Cell Cycle Proteins Fungal Proteins Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gibson S I
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Surosky R T
Tye B K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.11.5707-5720.1990
Published
1990-11-00
Pages
5707-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361340
Subset
IM
Grants
NIGMS NIH HHS · 2T32GM07273 · United States
NIGMS NIH HHS · GM34190 · United States
Databases
GENBANK
X53540
External Links
PubMed source DOI: 10.1128/mcb.10.11.5707-5720.1990 PMC Full Text View journal details
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