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PMID: 8763935 Published · ppublish English Journal Article

Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote.

Journal of bacteriology ·Vol. 178 ·No. 14 ·1996-07-00 ·Pages 4084-8

Frasch SC, Dworkin M

Abstract

Tyrosine phosphorylation is an extremely rare event in prokaryotes, occurring almost exclusively in multicellular eukaryotes. We have identified, for the first time, by the use of antiphosphotyrosine monoclonal antibody and Western blot (immunoblot) analysis, two tyrosine-phosphorylated membrane proteins in the multicellular prokaryote Myxococcus xanthus. The pattern of tyrosine phosphorylation was shown to change during development, indicating a possible role for this regulatory modification during two stages of development, i.e., aggregation and sporulation. Furthermore, the altered pattern of tyrosine phosphorylation observed in a variety of signaling mutants was shown to differ from that observed in the wild type, suggesting further the possible involvement of tyrosine phosphorylation during the development program.

MeSH Terms
Antibodies, Monoclonal Caseins/metabolism Cell Differentiation/genetics Membrane Proteins/metabolism Mutation Myxococcus xanthus/cytology,genetics,growth & development,metabolism Phosphorylation Phosphotyrosine/immunology,isolation & purification Prokaryotic Cells/metabolism Protein Hydrolysates/metabolism Protein-Tyrosine Kinases/metabolism Tyrosine/metabolism
Chemicals
Antibodies, Monoclonal Caseins Membrane Proteins Protein Hydrolysates trypticase-soy broth Phosphotyrosine Tyrosine Protein-Tyrosine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Frasch S C
Department of Microbiology, University of Minnesota, Minneapolis, 55455, USA.
Dworkin M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.14.4084-4088.1996
Published
1996-07-00
Pages
4084-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178164
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.14.4084-4088.1996 PMC Full Text View journal details
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