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PMID: 8808921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan.

Journal of bacteriology ·Vol. 178 ·No. 18 ·1996-09-00 ·Pages 5347-52

Mengin-Lecreulx D, van Heijenoort J, Park JT

Abstract

A gene, mpl, encoding UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelat e ligase was recognized by its amino acid sequence homology with murC as the open reading frame yjfG present at 96 min on the Escherichia coli map. The existence of such an enzymatic activity was predicted from studies indicating that reutilization of the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate occurred and accounted for well over 30% of new cell wall synthesis. Murein tripeptide ligase activity could be demonstrated in crude extracts, and greatly increased activity was produced when the gene was cloned and expressed under control of the trc promoter. A null mutant totally lacked activity but was viable, showing that the enzyme is not essential for growth.

MeSH Terms
Acetylmuramyl-Alanyl-Isoglutamine/analogs & derivatives,metabolism Amino Acid Sequence Cell Wall/metabolism Chromosome Mapping Escherichia coli/enzymology,genetics,growth & development Genes, Bacterial Molecular Sequence Data Mutation Peptide Synthases/genetics Peptidoglycan/metabolism Recombinant Proteins/biosynthesis Uridine Diphosphate N-Acetylmuramic Acid/metabolism
Chemicals
Peptidoglycan Recombinant Proteins Uridine Diphosphate N-Acetylmuramic Acid Acetylmuramyl-Alanyl-Isoglutamine muramylNAc-Ala-isoGln-Lys-tripeptide Peptide Synthases UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mengin-Lecreulx D
Unité de Recherche Associée 1131 du Centre National de la Recherche Scientifique, Université Paris-Sud, Orsay, France. [email protected]
van Heijenoort J
Park J T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.18.5347-5352.1996
Email
[email protected]
Published
1996-09-00
Pages
5347-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178350
Subset
IM
Databases
GENBANK
U14003, X52644
External Links
PubMed source DOI: 10.1128/jb.178.18.5347-5352.1996 PMC Full Text View journal details
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