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PMID: 8816469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Upstream binding factor stabilizes Rib 1, the TATA-binding-protein-containing Xenopus laevis RNA polymerase I transcription factor, by multiple protein interactions in a DNA-independent manner.

Molecular and cellular biology ·Vol. 16 ·No. 10 ·1996-10-00 ·Pages 5572-8

Bodeker M, Cairns C, McStay B

Abstract

Initiation of RNA polymerase I transcription in Xenopus laevis requires Rib 1 and upstream binding factor (UBF). UBF and Rib 1 combine to form a stable transcription complex on the Xenopus ribosomal gene promoter. Here we show that Rib 1 comprises TATA-binding protein (TBP) and TBP-associated factor components. Thus, Rib 1 is the Xenopus equivalent of mammalian SL 1. In contrast to SL 1, Rib 1 is an unstable complex that readily dissociates into TBP and associated components. We identify a novel function for UBF in stabilizing Rib 1 by multiple protein interactions. This stabilization occurs in solution in a DNA-independent manner. These results may partially explain the difference in UBF requirement between Xenopus and mammalian systems.

MeSH Terms
Amino Acid Sequence Animals Antibodies Binding Sites DNA/metabolism DNA-Binding Proteins/chemistry,metabolism Mammals Molecular Sequence Data Peptide Fragments/chemistry,immunology Pol1 Transcription Initiation Complex Proteins Protein Binding Protein Biosynthesis RNA Polymerase I/metabolism Recombinant Proteins/biosynthesis,chemistry,metabolism TATA Box TATA-Box Binding Protein Transcription Factors/biosynthesis,chemistry,metabolism Transcription, Genetic Xenopus Proteins Xenopus laevis
Chemicals
Antibodies DNA-Binding Proteins Peptide Fragments Pol1 Transcription Initiation Complex Proteins Recombinant Proteins Rib1 protein, Xenopus TATA-Box Binding Protein Transcription Factors Xenopus Proteins transcription initiation factor TIF-IB DNA RNA Polymerase I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bodeker M
Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Scotland.
Cairns C
McStay B
References (31)
31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.10.5572
Published
1996-10-00
Pages
5572-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231556
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/MCB.16.10.5572 PMC Full Text View journal details
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