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PMID: 8830771 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sorting by COP I-coated vesicles under interphase and mitotic conditions.

The Journal of cell biology ·Vol. 134 ·No. 6 ·1996-09-00 ·Pages 1411-25

Sönnichsen B, Watson R, Clausen H, Misteli T, Warren G

Abstract

COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N-acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when the vesicles were prepared under interphase or mitotic conditions showing that sorting was unaffected. The average density relative to starting membranes for resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%) provides clues to the function of COP I vesicles in transport through the Golgi apparatus.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins/analysis,metabolism Biological Transport/physiology Cell Compartmentation/physiology Golgi Apparatus/chemistry,enzymology Immunohistochemistry Interphase/physiology Intracellular Membranes/chemistry,enzymology Liver/cytology Mannosidases/analysis Microscopy, Immunoelectron Mitosis/physiology Molecular Sequence Data Proteins/metabolism Rats
Chemicals
Bacterial Proteins Proteins cop protein, Bacteria Mannosidases mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sönnichsen B
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Watson R
Clausen H
Misteli T
Warren G
References (76)
76 references, click to expand
  1. Concentration of intracellular hepatic apolipoprotein E in Golgi apparatus saccular distensions and endosomes.
    J Cell Biol. 1994 Dec;127(6 Pt 2):1859-69 PMID: 7806565
  2. Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.
    Cell. 1989 Feb 10;56(3):357-68 PMID: 2536591
  3. Kin recognition between medial Golgi enzymes in HeLa cells.
    EMBO J. 1994 Feb 1;13(3):562-74 PMID: 8313901
  4. Sorting of membrane proteins in the secretory pathway.
    Cell. 1993 Nov 19;75(4):603-5 PMID: 8242736
  5. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs.
    Science. 1994 Mar 18;263(5153):1629-31 PMID: 8128252
  6. Characterization of VIP36, an animal lectin homologous to leguminous lectins.
    J Cell Sci. 1996 Jan;109 ( Pt 1):271-6 PMID: 8834812
  7. Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum.
    Cell. 1989 Aug 25;58(4):707-18 PMID: 2527615
  8. Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus.
    J Cell Biol. 1994 Jul;126(1):41-52 PMID: 8027184
  9. Detergent influence on rat-liver galactosyltransferase activities towards different acceptors.
    Eur J Biochem. 1977 Jul 1;77(1):181-92 PMID: 198210
  10. COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast.
    Cell. 1995 Dec 29;83(7):1183-96 PMID: 8548805
  11. Folding and oligomerization of influenza hemagglutinin in the ER and the intermediate compartment.
    EMBO J. 1995 Apr 3;14(7):1340-8 PMID: 7729412
  12. Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect.
    J Cell Biol. 1976 Sep;70(3):608-21 PMID: 783172
  13. Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells.
    J Cell Biol. 1994 Dec;127(6 Pt 1):1557-74 PMID: 7798312
  14. Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme.
    J Biol Chem. 1991 Sep 5;266(25):16876-85 PMID: 1885615
  15. Biosynthetic protein transport in the secretory pathway.
    Curr Opin Cell Biol. 1989 Aug;1(4):648-54 PMID: 2516740
  16. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway.
    Cell. 1990 Jun 29;61(7):1349-57 PMID: 2194670
  17. A novel class of clathrin-coated vesicles budding from endosomes.
    J Cell Biol. 1996 Jan;132(1-2):21-33 PMID: 8567724
  18. ERGIC-53, a membrane protein of the endoplasmic reticulum-Golgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells.
    J Biol Chem. 1995 Feb 24;270(8):3551-3 PMID: 7876089
  19. The Golgi complex: in vitro veritas?
    Cell. 1992 Mar 6;68(5):829-40 PMID: 1547485
  20. Three-dimensional electron microscopy: structure of the Golgi apparatus.
    Eur J Cell Biol. 1990 Apr;51(2):189-200 PMID: 2190832
  21. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin.
    Cell. 1991 Feb 8;64(3):649-65 PMID: 1840503
  22. Localization of O-glycan initiation, sphingomyelin synthesis, and glucosylceramide synthesis in Vero cells with respect to the endoplasmic reticulum-Golgi intermediate compartment.
    J Biol Chem. 1994 Feb 11;269(6):4035-41 PMID: 8307959
  23. Mitotic disassembly of the mammalian Golgi apparatus.
    Trends Cell Biol. 1995 Nov;5(11):413-6 PMID: 14732040
  24. Glycosyl transferases of baby-hamster-kidney (BHK) cells and ricin-resistant mutants. N-glycan biosynthesis.
    Eur J Biochem. 1981 Jul;117(2):275-84 PMID: 6456144
  25. Four secretory proteins synthesized by hepatocytes are transported from endoplasmic reticulum to Golgi complex at different rates.
    EMBO J. 1984 Jan;3(1):147-52 PMID: 6538481
  26. Immunocytochemical localization of albumin in the secretory apparatus of rat liver parenchymal cells.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4970-4 PMID: 7029527
  27. Heterogeneous distribution of filipin--cholesterol complexes across the cisternae of the Golgi apparatus.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):293-7 PMID: 7017713
  28. COP-coated vesicles are involved in the mitotic fragmentation of Golgi stacks in a cell-free system.
    J Cell Biol. 1994 Apr;125(2):269-82 PMID: 8163545
  29. The trans Golgi network: sorting at the exit site of the Golgi complex.
    Science. 1986 Oct 24;234(4775):438-43 PMID: 2945253
  30. The pathway of Golgi cluster formation in okadaic acid-treated cells.
    J Struct Biol. 1995 Nov-Dec;115(3):318-30 PMID: 8573473
  31. Distribution and processing of the polymeric immunoglobulin receptor in the rat hepatocyte: morphological and biochemical characterization of subcellular fractions.
    J Histochem Cytochem. 1986 Jan;34(1):17-23 PMID: 3941264
  32. Transport into and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase.
    J Cell Biol. 1994 Nov;127(3):641-52 PMID: 7962049
  33. An investigation of the role of transmembrane domains in Golgi protein retention.
    EMBO J. 1995 Oct 2;14(19):4695-704 PMID: 7588599
  34. An N-terminal double-arginine motif maintains type II membrane proteins in the endoplasmic reticulum.
    EMBO J. 1994 Apr 1;13(7):1696-705 PMID: 8157008
  35. Stepwise assembly of functionally active transport vesicles.
    Cell. 1993 Dec 3;75(5):1015-25 PMID: 8252615
  36. A C-terminal signal prevents secretion of luminal ER proteins.
    Cell. 1987 Mar 13;48(5):899-907 PMID: 3545499
  37. A putative novel class of animal lectins in the secretory pathway homologous to leguminous lectins.
    Cell. 1994 Jun 3;77(5):625-6 PMID: 8205612
  38. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics.
    Curr Biol. 1994 Mar 1;4(3):220-33 PMID: 7922327
  39. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
    Cell. 1994 Dec 30;79(7):1199-207 PMID: 8001155
  40. Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells.
    J Cell Biol. 1993 Jan;120(1):5-13 PMID: 8416995
  41. A role for tubular networks and a COP I-independent pathway in the mitotic fragmentation of Golgi stacks in a cell-free system.
    J Cell Biol. 1995 Sep;130(5):1027-39 PMID: 7657690
  42. Determination of lipid phosphorus in the nanomolar range.
    Anal Biochem. 1970 Jul;36(1):225-6 PMID: 5482631
  43. Assembly of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1985;54:631-64 PMID: 3896128
  44. The bulk-flow hypothesis: not quite the end.
    Trends Cell Biol. 1995 Jan;5(1):9-13 PMID: 14731423
  45. Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor.
    J Cell Biol. 1993 Jan;120(2):325-38 PMID: 8380600
  46. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  47. Protein sorting by transport vesicles.
    Science. 1996 Apr 12;272(5259):227-34 PMID: 8602507
  48. Newly synthesized hepatocyte plasma membrane proteins are transported in transcytotic vesicles in the bile duct-ligated rat.
    Gastroenterology. 1993 Aug;105(2):554-71 PMID: 8335210
  49. Subcellular organization of glycosylation in mammalian cells.
    Biochim Biophys Acta. 1987 Oct 5;906(3):405-36 PMID: 3307920
  50. A comparison of the transmembrane domains of Golgi and plasma membrane proteins.
    Biochem Soc Trans. 1995 Aug;23(3):527-30 PMID: 8566407
  51. The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi.
    EMBO J. 1995 Apr 3;14(7):1329-39 PMID: 7729411
  52. Scale formation in algae.
    J Electron Microsc Tech. 1991 Feb;17(2):165-78 PMID: 2013819
  53. Characterization of a 58 kDa cis-Golgi protein in pancreatic exocrine cells.
    J Cell Sci. 1992 Oct;103 ( Pt 2):321-33 PMID: 1478936
  54. Molecular cloning and expression of a 58-kDa cis-Golgi and intermediate compartment protein.
    J Biol Chem. 1996 Feb 23;271(8):4031-7 PMID: 8626736
  55. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  56. Sphingolipid transport in mitotic HeLa cells.
    J Biol Chem. 1992 Dec 5;267(34):24906-11 PMID: 1447226
  57. Density of newly synthesized plasma membrane proteins in intracellular membranes II. Biochemical studies.
    J Cell Biol. 1984 Jun;98(6):2142-7 PMID: 6563038
  58. Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus.
    J Cell Biol. 1988 Nov;107(5):1643-53 PMID: 3182932
  59. Evidence for recycling of the resident medial/trans Golgi enzyme, N-acetylglucosaminyltransferase I, in ldlD cells.
    J Biol Chem. 1995 Oct 20;270(42):25057-63 PMID: 7559636
  60. Binding of the vesicle docking protein p115 to Golgi membranes is inhibited under mitotic conditions.
    J Biol Chem. 1996 Jul 19;271(29):17304-11 PMID: 8663393
  61. Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum.
    Cell. 1992 Jan 24;68(2):353-64 PMID: 1310258
  62. Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
    EMBO J. 1990 Oct;9(10):3153-62 PMID: 2120038
  63. Coat proteins in intracellular membrane transport.
    Curr Opin Cell Biol. 1994 Aug;6(4):533-7 PMID: 7986530
  64. Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates.
    Nature. 1983 Jul 7-13;304(5921):80-3 PMID: 6866094
  65. Microsomal triglyceride transfer protein: a protein complex required for the assembly of lipoprotein particles.
    Trends Cell Biol. 1995 Aug;5(8):317-21 PMID: 14732096
  66. Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides.
    J Cell Sci. 1995 Apr;108 ( Pt 4):1617-27 PMID: 7615680
  67. A microtubule-binding protein associated with membranes of the Golgi apparatus.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2229-39 PMID: 3536963
  68. Cyclin A-mediated inhibition of intra-Golgi transport requires p34cdc2.
    FEBS Lett. 1993 Dec 28;336(3):549-54 PMID: 8282125
  69. Localization of a yeast early Golgi mannosyltransferase, Och1p, involves retrograde transport.
    J Cell Biol. 1996 Mar;132(6):985-98 PMID: 8601597
  70. A mitotic form of the Golgi apparatus in HeLa cells.
    J Cell Biol. 1987 Apr;104(4):865-74 PMID: 3104351
  71. Fragmentation and partitioning of the Golgi apparatus during mitosis in HeLa cells.
    EMBO J. 1987 Nov;6(11):3239-46 PMID: 3428259
  72. Inhibition of intra-Golgi transport in vitro by mitotic kinase.
    J Biol Chem. 1993 Feb 25;268(6):4050-4 PMID: 8382687
  73. A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.
    Cell. 1986 Jul 18;46(2):171-84 PMID: 2872969
  74. Scale formation in chrysophycean algae. I. Cellulosic and noncellulosic wall components made by the Golgi apparatus.
    J Cell Biol. 1970 May;45(2):246-71 PMID: 5513606
  75. A brefeldin A-like phenotype is induced by the overexpression of a human ERD-2-like protein, ELP-1.
    Cell. 1992 May 15;69(4):625-35 PMID: 1316805
  76. Sorting and retrieval between the endoplasmic reticulum and Golgi apparatus.
    Curr Opin Cell Biol. 1995 Aug;7(4):530-5 PMID: 7495573
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.134.6.1411
Published
1996-09-00
Pages
1411-25
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120996
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.134.6.1411 PMC Full Text View journal details
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