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PMID: 8847994 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regional brain effects of sodium azide treatment on cytochrome oxidase activity: a quantitative histochemical study.

Metabolic brain disease ·Vol. 10 ·No. 4 ·1995-12-00 ·Pages 303-20

Cada A, Gonzalez-Lima F, Rose GM, Bennett MC

Abstract

The objective of the present study was to determine if regional variation in brain cytochrome oxidase activity was observed following systemic administration of sodium azide. An image analysis system calibrated with internal standards of known cytochrome oxidase activity was used to quantify cytochrome oxidase in histochemically stained brain sections. Rats receiving chronic infusion of sodium azide (400 micrograms/hr), which were sacrificed after two weeks, showed a substantial decrease in brain cytochrome oxidase activity over those infused with saline. All of the 22 regions sampled from telencephalic, diencephalic, and mesencephalic levels, showed a significant activity reduction which ranged between 26% and 37%. The regions that appeared significantly more vulnerable to the sodium azide effects were the mesencephalic reticular formation and the central amygdala, which displayed the largest decrease in activity. In addition, interregional correlations of activity showed a deeply modified pattern of correlative metabolic activity between hippocampal, amygdaloid and cortical areas after azide treatment. The regional effects found were consistent with azide-induced learning and memory dysfunctions.

MeSH Terms
Animals Azides/toxicity Brain/drug effects,enzymology Brain Chemistry/drug effects Brain Mapping Carbon Monoxide/metabolism Electron Transport Complex IV/antagonists & inhibitors,metabolism Enzyme Inhibitors/toxicity Histocytochemistry Image Processing, Computer-Assisted Male Rats Rats, Sprague-Dawley Sodium Azide
Chemicals
Azides Enzyme Inhibitors Carbon Monoxide Sodium Azide Electron Transport Complex IV
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cada A
Department of Psychology, University of Texas at Austin, Texas, USA.
Gonzalez-Lima F
Rose G M
Bennett M C
References (40)
40 references, click to expand
  1. Oxidative damage to mitochondrial DNA is increased in Alzheimer's disease.
    Ann Neurol. 1994 Nov;36(5):747-51 PMID: 7979220
  2. The effect of impulse blockage on cytochrome oxidase activity in the cat visual system.
    Brain Res. 1983 Feb 21;261(2):185-93 PMID: 6299464
  3. Thiamine-dependent enzyme changes in temporal cortex of patients with Alzheimer's disease.
    Metab Brain Dis. 1990 Dec;5(4):179-84 PMID: 2087217
  4. Cortical cytochrome oxidase activity is reduced in Alzheimer's disease.
    J Neurochem. 1994 Dec;63(6):2179-84 PMID: 7964738
  5. Structural modeling of functional neural pathways mapped with 2-deoxyglucose: effects of acoustic startle habituation on the auditory system.
    Brain Res. 1991 May 3;547(2):295-302 PMID: 1884204
  6. Regional brain effects of sodium azide treatment on cytochrome oxidase activity: a quantitative histochemical study.
    Metab Brain Dis. 1995 Dec;10(4):303-20 PMID: 8847994
  7. Cytochrome oxidase activity in the auditory system of the mouse: a qualitative and quantitative histochemical study.
    Neuroscience. 1994 Nov;63(2):559-78 PMID: 7891865
  8. Network analysis of functional auditory pathways mapped with fluorodeoxyglucose: associative effects of a tone conditioned as a Pavlovian excitor or inhibitor.
    Brain Res. 1993 Nov 5;627(1):129-40 PMID: 8293293
  9. Functional mapping of the rat brain during drinking behavior: a fluorodeoxyglucose study.
    Physiol Behav. 1993 Sep;54(3):605-12 PMID: 8415957
  10. Cytochrome oxidase: an endogenous metabolic marker for neuronal activity.
    Trends Neurosci. 1989 Mar;12(3):94-101 PMID: 2469224
  11. An optimized method for determining cytochrome oxidase activity in brain tissue homogenates.
    J Neurosci Methods. 1993 Dec;50(3):309-19 PMID: 8152242
  12. Cytochrome oxidase deficiency in Alzheimer's disease.
    Neurology. 1990 Aug;40(8):1302-3 PMID: 2166249
  13. Changes in endogenous enzymatic reactivity to DAB induced by neuronal inactivity.
    Brain Res. 1978 Feb 3;141(1):185-92 PMID: 414822
  14. Cytochrome oxidase inhibition: a novel animal model of Alzheimer's disease.
    J Geriatr Psychiatry Neurol. 1992 Apr-Jun;5(2):93-101 PMID: 1317179
  15. Chronic sodium azide treatment impairs learning of the Morris water maze task.
    Behav Neural Biol. 1992 Jul;58(1):72-5 PMID: 1417674
  16. Do defects in mitochondrial energy metabolism underlie the pathology of neurodegenerative diseases?
    Trends Neurosci. 1993 Apr;16(4):125-31 PMID: 7682343
  17. Long-term changes in regional brain cytochrome oxidase activity induced by electroconvulsive treatment in rats.
    Brain Res. 1993 Mar 5;605(1):1-8 PMID: 8385539
  18. Intercorrelations of regional cerebral glucose metabolic rates in Alzheimer's disease.
    Brain Res. 1987 Mar 31;407(2):294-306 PMID: 3494486
  19. Technical considerations on the use of horseradish peroxidase as a neuronal marker.
    Neuroscience. 1977;2(1):141-5 PMID: 917271
  20. Age-related modifications of cytochrome C oxidase activity in discrete brain regions.
    Mech Ageing Dev. 1990 Aug;55(2):171-80 PMID: 2172666
  21. Differential expression of cytochrome oxidase (COX) genes in different regions of monkey brain.
    J Neurosci Res. 1992 Jul;32(3):415-23 PMID: 1279190
  22. Reduced platelet cytochrome c oxidase activity in Alzheimer's disease.
    Neurology. 1994 Jun;44(6):1086-90 PMID: 8208406
  23. Network interactions among limbic cortices, basal forebrain, and cerebellum differentiate a tone conditioned as a Pavlovian excitor or inhibitor: fluorodeoxyglucose mapping and covariance structural modeling.
    J Neurophysiol. 1994 Oct;72(4):1717-33 PMID: 7823097
  24. Energy metabolism in disorders of the nervous system.
    Rev Neurol (Paris). 1988;144(10):543-63 PMID: 2973643
  25. Modified technique for cytochrome oxidase histochemistry: increased staining intensity and compatibility with 2-deoxyglucose autoradiography.
    J Neurosci Methods. 1987 Jan;19(1):1-10 PMID: 2434810
  26. Quantitative histochemistry of cytochrome oxidase in rat brain.
    Neurosci Lett. 1991 Feb 25;123(2):251-3 PMID: 1709270
  27. Brain cytochrome oxidase in Alzheimer's disease.
    J Neurochem. 1992 Aug;59(2):776-9 PMID: 1321237
  28. Electron transport chain defects in Alzheimer's disease brain.
    Neurology. 1994 Jun;44(6):1090-6 PMID: 8208407
  29. Quantitative mapping of cytochrome oxidase activity in the central auditory system of the gerbil: a study with calibrated activity standards and metal-intensified histochemistry.
    Brain Res. 1994 Oct 10;660(1):34-49 PMID: 7828000
  30. Coordination of ATP production and consumption in brain: parallel regulation of cytochrome oxidase and Na+, K(+)-ATPase.
    Neurosci Lett. 1992 Apr 13;138(1):188-92 PMID: 1328957
  31. Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB).
    J Cell Biol. 1968 Jul;38(1):1-14 PMID: 4300067
  32. Impairment in mitochondrial cytochrome oxidase gene expression in Alzheimer disease.
    Brain Res Mol Brain Res. 1994 Jul;24(1-4):336-40 PMID: 7968373
  33. Ascending reticular activating system in the rat: a 2-deoxyglucose study.
    Brain Res. 1985 Sep 30;344(1):70-88 PMID: 4041870
  34. Ultramicrospectrophotometric determination of cytochrome oxidase for quantitative histochemistry.
    J Biol Chem. 1953 Sep;204(1):295-306 PMID: 13084602
  35. Changes in the visual system of monocularly sutured or enucleated cats demonstrable with cytochrome oxidase histochemistry.
    Brain Res. 1979 Jul 27;171(1):11-28 PMID: 223730
  36. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  37. Quantitative histochemistry of acetylcholinesterase in rat and human brain postmortem.
    J Neurosci Methods. 1986 Mar;16(1):39-45 PMID: 2422501
  38. Brain cytochrome oxidase: purification, antibody production, and immunohistochemical/histochemical correlations in the CNS.
    J Neurosci. 1989 Nov;9(11):3884-98 PMID: 2555458
  39. Functional interactions in the brain: use of correlations between regional metabolic rates.
    J Cereb Blood Flow Metab. 1991 Mar;11(2):A114-20 PMID: 1997479
  40. Structural modeling of functional visual pathways mapped with 2-deoxyglucose: effects of patterned light and footshock.
    Brain Res. 1992 Apr 24;578(1-2):75-86 PMID: 1511292
Article Info
Journal
Metabolic brain disease
Abbr.
Metab Brain Dis
ISSN
0885-7490
DOI
10.1007/BF02109361
Published
1995-12-00
Pages
303-20
Language
English
Region
United States
NLM ID
8610370
Subset
IM
Grants
NIA NIH HHS · AG10755 · United States
NIMH NIH HHS · R0 MH43353 · United States
External Links
PubMed source DOI: 10.1007/BF02109361 View journal details
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